Browsing by Author "Brede, H. D."

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    An appraisal of the uricult dip slide method in the diagnosis of urinary infections
    (HMPG, 1973-05) Finlayson, M. H.; Coates, J. K.; Brede, H. D.; Mitchell, P.
    Uricult dip slide urine cultures were compared with standard laboratory plate cultures. Good agreement of bacterial counts was obtained after incubation at 37°C but not at room temperature. Tests of therapeutic activity of various drugs on the commoner organisms producing urinary infections, were done. The results suggested that such tests had no positive value. Uricult dip slides should be of value as a suitable transport and diagnostic medium for the diagnosis of urinary tract infections.
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    Detection of biological false positive syphilis serum reactions
    (Health & Medical Publishing Group, 1974) Brede, H. D.; Willey, K. D. F.; Kindermann, R. A.; Finlayson, M. H.
    A comparative evaluation of reagin tests (Wassermann, VDRL, RPR) and fluorescent treponema antibody absorption tests (FTA ABS) performed on blood specimens from 5,271 persons (2,493 pregnant women, 1,130 apparently healthy prospective employees, 1,345 newborn babies and 303 leprosy patients) showed that 17.2% of the pregnant women, 11.95% of the prospective employees, 19.0% of the newborn babies, and 27.2% of the leprosy patients gave positive reactions in one or more of the tests. The majority of specimens were from Cape Colored patients. FTA ABS tests allowed the exclusion as biological false positives of ± 30% of the pregnant 'positive reactors', of ± 37% of the prospective employees, 40.2% of the newborn babies, but only 1% of the leprosy patients. The FTA ABS test, therefore, deserves wide acceptance as the standard by which the diagnosis of syphilis is confirmed and false positive reactions are defined, although non specificity in pregnancies, intracellular infections and auto immune diseases occurs. The classical reagin tests are of value in the control of treatment.
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    Diagnosis and incidence of Neisseria gonorrhoeae in Cape Coloured females in the Western Cape : laboratory aspects
    (Health & Medical Publishing Group, 1974) Finlayson, M. H.; Gibbs, B.; Brede, H. D.
    Specimens were taken, using carbon impregnated swabs, from the cervix, urethra and rectrum of 945 Cape Coloured gynaecological patients, and from the cervix only of 1,276 pregnant Cape Coloured women. These specimens were submitted to the laboratory in a modified Stuart transport medium and cultured on Thayer Martin medium. N. gonorrhoeae was cultured in 5.3% of the specimens from the gynaecological patients and in 5.3% of specimens from the pregnant women. All cultures showed type I or II colony pattern. No strains showed resistance to any of the antibiotics tested.
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    Escherichia coli serotypes associated with urinary tract infections in the Western Cape
    (Health & Medical Publishing Group, 1974) Brede, H. D.; Coldrey, N. A.; Coates, J. K.; Finlayson, M. H.
    Examination of 3,261 specimens of urine from patients with urinary tract infections led to the isolation of E. coli types from 933 samples (28.6%). Serological typing revealed 222 isolates (23.8%) as urinary or as enteropathogenic serotypes. The rest were untypable. The following urinary types were identified: O 1, O 2, O 4, O 5, O 6, O 7, O 9, O 11, O 18, O 39, O 75, the commonest being O 6, O 4, and O 75. Enteropathogenic types were O 26-B6, O 55-B5, O 86-B7, O 111-B4, O 112-B11, O 119-B14, O 124-B17, O 125-B15, O 126-B16, O 127-B8, O 128-B12, and O 142-B. Types O 112 and O 111 were the most prevalent. The 1973 pattern of urinary E. coli infections in the Western Cape differs from that found in other parts of the world. Type O 6 is most prevalent, followed by O 4, and O 75. The last is the most common type north of the equator. Other types were far less frequent. Crossreactivity between 14 V. cholerae strains and E. coli O 39 antiserum was proved, suggesting similarities between the enterotoxins of V. cholerae and pathogenic E. coli strains.
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    Prevention of food poisoning in hospital patients
    (Health & Medical Publishing Group, 1974) Horwitz, B. M.; Finlayson, M. H.; Brede, H. D.
    Bacteriological investigations of raw and cooked foods and of food handlers in abattoirs, food factories and hospital kitchens show that they are potential sources of food poisoning organisms. The use of reheated (reconstituted) frozen foods is recommended as an ideal means of preventing food poisoning among hospital patients.