Browsing by Author "Addison, Matthew F."

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    Characterisation of Metarhizium majus (Hypocreales : Clavicipitaceae) isolated from the Western Cape Province, South Africa
    (Public Library of Science, 2021-03-19) Mathulwe, Letodi L.; Jacobs, Karin; Malan, Antoinette P.; Birkhofer, Klaus; Addison, Matthew F.; Addison, Pia
    Entomopathogenic fungi (EPF) are important soil-dwelling entomopathogens, which can be used as biological control agents against pest insects. EPF are capable of causing lethal epizootics in pest insect populations in agroecosystems. During a survey of the orchard soil at an organic farm, different EPF species were collected and identified to species level, using both morphological and molecular techniques. The EPF were trapped from soil samples taken from an apricot orchard. The traps, which were baited in the laboratory, used susceptible host insects, including the last-instar larvae of Galleria mellonella (wax moth larvae) and Tenebrio molitor (mealworm larvae). The potential pathogenicity of the local Metarhizium majus isolate was tested and verified using susceptible laboratory-reared last-instar T. molitor larvae. The identification of the M. majus isolated from South African soil was verified using both morphological and molecular techniques. The occurrence of M. majus in the South African soil environment had not previously been reported.
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    Effect of humidity and a superabsorbent polymer formulation on the efficacy of Heterorhabditis zealandica (Rhabditida: Heterorhabditidae) to control codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae)
    (Taylor & Francis, 2013-12) De Waal, Jeanne Y.; Malan, Antoinette P.; Addison, Matthew F.
    Adequate moisture levels are required for nematode survival and subsequent efficacy as entomopathogens. Formulation of nematodes aimed at aboveground applications may assist in maintaining such moisture levels. In this study, we report the effects of a superabsorbent polymer formulation, Zeba® on the performance of an entomopathogenic nematode, Heterorhabditis zealandica Poinar, for controlling diapausing codling moth, Cydia pomonella (L.) larvae in cryptic habitats on trees. Water activity (aw-value) on bark was considered to be an indication of moisture levels on trees in cryptic habitats where codling moth larvae are known to occur, thereby influencing nematode efficacy. H. zealandica was only able to infect codling moth larvae at aw≥0.92, with aw50=0.94 and aw90=0.96.
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    Efficacy of entomopathogenic nematodes (Rhabditida : Heterorhabditidae and Steinernematidae) against codling moth, Cydia pomonella (Lepidoptera: Tortricidae) in temperate regions
    (Taylor & Francis, 2011-09) De Waal, Jeanne Y.; Malan, Antoinette P.; Addison, Matthew F.
    The biocontrol potential of South African isolates of Heterorhabditis zealandica, Steinernema citrae, S. khoisanae, S. yirgalemense, and Steinernema sp., was evaluated against codling moth, Cydia pomonella. Codling moth was susceptible to all six nematode isolates at a concentration of 50 infective juveniles/insect (78-100% mortality). Low temperatures (10 h at 17ºC; 14 h at 12ºC) negatively affected larvicidal activity (≤3%) for all isolates. All tested isolates were most effective at higher levels of water activity (aw=1). The average aw50-values for all isolates tested was 0.94 (0.93-0.95), except S. khoisanae 0.97 (0.97-0.98). Regarding host-seeking ability, no positive attraction to host cues could be detected amongst isolates, except for H. zealandica. Three of the isolates, H. zealandica, S. khoisanae, and the undescribed Steinernema sp., were selected for field-testing and proven to be effective (mortality >50%). Insect containment methods used during field experimentation was shown to influence larvacidal activity, as different levels of mortality were obtained using various containment methods (wooden planks vs. pear tree logs vs. mesh cages). Pear tree logs were impractical. Predictive equations were subsequently developed, enabling future trials to be conducted using either planks or cages, enabling the prediction of the expected level of control on tree logs. All tested isolates therefore showed a certain degree of biological control potential, however, none of the experiments showed clear efficacy-differences amongst isolates. The study highlighted the importance of environmental factors to ensure the successful application of these nematodes for the control of diapausing codling moth larvae in temperate regions.
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    Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the southwestern parts of South Africa
    (SA ePublications, 2006) Malan, Antoinette P.; Nguyen, Khuong B.; Addison, Matthew F.
    Soil samples were collected in the southwestern parts of South Africa to obtain entomopathogenic nematodes of the families Steinernematidae and Heterorhabditidae. In total, 498 samples were randomly taken from cultivated and uncultivated habitats, including deciduous fruit orchards, vineyards and natural vegetation. Entomopathogenic nematodes were isolated from 36 samples (7 %) by baiting with larvae of Galleria mellonella (greater wax moth). Heterorhabditis was the dominant genus isolated, while Steinernema was rare. The most common species was Heterorhabditis bacteriophora Other species identified were Heterorhabditis zealandica and Steinernema khoisanae. The isolation of H. zealandica represents a new record for South Africa, whereasS. khoisanae has thus far been recorded only from South Africa.
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    Key elements in the successful control of diapausing codling moth, Cydia pomonella (Lepidoptera: Tortricidae) in wooden fruit bins with a South African isolate of Heterorhabditis zealandica (Rhabditida: Heterorhabditidae)
    (Taylor & Francis, 2010-01) De Waal, Jeanne Y.; Malan, Antoinette P.; Levings, John; Addison, Matthew F.
    The non-insecticidal control strategies currently being implemented in South African orchards for the control of codling moth, Cydia pomonella (L.) may be hampered bywooden fruit bins being infested with diapausing codling moth larvae, acting as a potential source of re-infestation. Key factors contributing to the success or failure of an entomopathogenic nematode application were investigated using the SF 41 isolate of Heterorhabditis zealandica in laboratory bioassays with wooden minibins. Under operational conditions, an application rate of 100 IJs/mL (LD9=102 IJs/mL) effectively controlled codling moth larvae in these bins, and for further laboratory bioassays, the LD50 value of 18 IJs/mL (≈25 IJs/mL) was identified as the discriminating dosage. Maximum mortality was attained when bins were pre-wet for at least 1 min (>90% RH) and maintained at maximum humidity (>95% RH) post-treatment for at least 3 days (LT90=73 h), to ensure nematode survival and subsequent satisfactory infection of diapausing codling moth larvae. Tarping bins achieved the desired high level of humidity required. Furthermore, adjuvants (specifically Reverseal 10™) also improved an application. The study conclusively illustrated that if all the above-mentioned conditions are met, H. zealandica has the potential to successfully disinfest wooden fruit bins of codling moth.