Clinical importance of a micro-assay for the evaluation of sperm acrosome reaction using homologous zona pellucida

Simple item page
dc.contributor.authorEsterhuizen A.D.
dc.contributor.authorFranken D.R.
dc.contributor.authorLourens J.G.H.
dc.contributor.authorVan Rooyen L.H.
dc.date.accessioned2011-05-15T16:15:25Z
dc.date.available2011-05-15T16:15:25Z
dc.date.issued2001
dc.description.abstractThis study aimed to develop an acrosome reaction assay using microvolumes of solubilized human zonae pellucidae among 35 couples attending an in vitro fertilization programme. The sperm morphology of the men was classified as g-pattern (5-14% normal forms) and/or normal pattern (>14% normal forms). All the couples had a history of repeated poor or failed in vitro fertilization rates from previous attempts. A zona-induced acrosome reaction test was performed using homologous 0.25 zona pellucida μl-1 incubated with spermatozoa to induce the acrosome reaction. Acrosome reactions were measured with FITC-PSA staining, and expressed as the difference between zona-induced and spontaneous acrosome reaction spermatozoa. The results indicated that microvolumes of solubilized human zona pellucida could successfully be used to determine the acrosome reaction status of spermatozoa. The results were compared with in vitro fertilization rates of metaphase II oocytes, and analysed with the receiver operating characteristics curve. Receiver operating characteristics analyses divided the patients into two groups: i.e. zona-induced acrosome reaction <15% and >15%. The sensitivity and specificity for zona-induced acrosome reaction results versus fertilization were 93% and 100%, respectively. The correlation coefficient between zona-induced acrosome reaction and in vitro fertilization was r=0.94 (P<0.0001). Zona-induced acrosome reaction data can be used as an indictor for fertilization failure, thus helping clinicians to refine the therapeutic approach for infertile couples prior to the onset of the treatment.
dc.description.versionReview
dc.identifier.citationAndrologia
dc.identifier.citation33
dc.identifier.citation2
dc.identifier.issn03034569
dc.identifier.urihttp://hdl.handle.net/10019.1/13334
dc.subjectfluorescein isothiocyanate
dc.subjectfluorescent dye
dc.subjectlectin
dc.subjectpea lectin
dc.subjectplant lectin
dc.subjectacrosome reaction
dc.subjectarticle
dc.subjectcongenital malformation
dc.subjectfemale
dc.subjectfertilization in vitro
dc.subjecthuman
dc.subjectinfertility
dc.subjectmale
dc.subjectpathophysiology
dc.subjectphysiology
dc.subjectroc curve
dc.subjectspermatozoon
dc.subjectzona pellucida
dc.subjectAcrosome Reaction
dc.subjectFemale
dc.subjectFertilization in Vitro
dc.subjectFluorescein-5-isothiocyanate
dc.subjectFluorescent Dyes
dc.subjectHumans
dc.subjectInfertility
dc.subjectLectins
dc.subjectMale
dc.subjectPlant Lectins
dc.subjectROC Curve
dc.subjectSpermatozoa
dc.subjectZona Pellucida
dc.titleClinical importance of a micro-assay for the evaluation of sperm acrosome reaction using homologous zona pellucida
dc.typeReview
Files
Collections
Stellenbosch University - Scopus Tygerberg Hospital Publications