Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization

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dc.contributor.authorSun, Jinfengen_ZA
dc.contributor.authorTian, Kangmingen_ZA
dc.contributor.authorWang, Jieen_ZA
dc.contributor.authorDong, Zixingen_ZA
dc.contributor.authorLiu, Xiaoguangen_ZA
dc.contributor.authorPermaul, Kugenthirenen_ZA
dc.contributor.authorSingh, Surenen_ZA
dc.contributor.authorPrior, Bernard A.en_ZA
dc.contributor.authorWang, Zhengxiangen_ZA
dc.date.accessioned2018-05-07T05:36:02Z
dc.date.available2018-05-07T05:36:02Z
dc.date.issued2018-05-02
dc.date.updated2018-05-06T03:38:26Z
dc.descriptionCITATION: Sun, Jinfeng, et al. 2018. Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization. Microbial Cell Factories, 17:66, doi:10.1186/s12934-018-0915-x.en_ZA
dc.descriptionThe original publication is available at https://microbialcellfactories.biomedcentral.comen_ZA
dc.description.abstractBackground: Lignocellulosic ethanol could offer a sustainable source to meet the increasing worldwide demand for fuel. However, efficient and simultaneous metabolism of all types of sugars in lignocellulosic hydrolysates by ethanolproducing strains is still a challenge. Results: An engineered strain Escherichia coli B0013-2021HPA with regulated glucose utilization, which could use all monosaccharides in lignocellulosic hydrolysates except glucose for cell growth and glucose for ethanol production, was constructed. In E. coli B0013-2021HPA, pta-ackA, ldhA and pflB were deleted to block the formation of acetate, lactate and formate and additional three mutations at glk, ptsG and manZ generated to block the glucose uptake and catabolism, followed by the replacement of the wild-type frdA locus with the ptsG expression cassette under the control of the temperature-inducible λ pR and pL promoters, and the final introduction of pEtac-PA carrying Zymomonas mobilis pdc and adhB for the ethanol pathway. B0013-2021HPA was able to utilize almost all xylose, galactose and arabinose but not glucose for cell propagation at 34 °C and converted all sugars to ethanol at 42 °C under oxygenlimited fermentation conditions. Conclusions: Engineered E. coli strain with regulated glucose utilization showed efficient metabolism of mixed sugars in lignocellulosic hydrolysates and thus higher productivity of ethanol production.en_ZA
dc.description.urihttps://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-018-0915-x
dc.description.versionPublisher's versionen_ZA
dc.format.extent8 pages : illustrationsen_ZA
dc.identifier.citationSun, Jinfeng, et al. 2018. Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization. Microbial Cell Factories, 17:66, doi:10.1186/s12934-018-0915-xen_ZA
dc.identifier.issn1475-2859 (online)
dc.identifier.otherdoi:10.1186/s12934-018-0915-x
dc.identifier.urihttp://hdl.handle.net/10019.1/103977
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights.holderAuthor retains copyrighten_ZA
dc.subjectEthanol productionen_ZA
dc.subjectLignocellulosic hydrolysateen_ZA
dc.subjectEscherichia colien_ZA
dc.subjectGlucose utilizationen_ZA
dc.titleImproved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilizationen_ZA
dc.typeArticleen_ZA
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