Steep gradients of inert substances as supports for precipitin reactions

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dc.contributor.authorPolson A.
dc.contributor.authorMaass R.
dc.date.accessioned2011-05-15T16:16:37Z
dc.date.available2011-05-15T16:16:37Z
dc.date.issued1989
dc.description.abstractPrecipitin reactions were conducted in the wells of micro titer plates and the light 'absorbance' at 405nm plotted as a function of the neg log2 dilutions of the antigens using the Titertek Multiscan apparatus. The procedure followed was to incorporate antibody in 20% sucrose and to diffuse the mixture into serial two-fold dilutions of antigen overlaid on the sugar-antibody columns in the wells. Where the antigen met its antibody in equivalence precipitin bands formed. The bands of precipitates remained suspended. The precipitates formed by mixing the antibody sugar mixture with the serial two-fold dilutions of the antigen were also recorded in the Titertek Multiscan apparatus. Tobacco mosaic virus and its IgY type antibody produced simoidal precipitin curves with the layering as well as the mixing techniques. The haemocyanin of the whelk and its IgY antibody yielded a precipitin diagram with the layering technique which gave evidence to three antigens. The three antigens in the haemocyanin was confirmed by two dimentional Laurell-electrophoresis. Semi-purified human colostral dimeric IgA and its IgY antibody produced a precipitin diagram which showed evidence of four or five antigens. With the mixing technique a single precipitin maximum was obtained preceded by a prozone. Rabbit Ig and its IgY antibody gave a precipitin curve with two maxima. The precipitin curve obtained when the reagents were mixed had one peak preceded by a prozone.
dc.description.versionArticle
dc.identifier.citationImmunological Investigations
dc.identifier.citation18
dc.identifier.citation6
dc.identifier.issn08820139
dc.identifier.urihttp://hdl.handle.net/10019.1/13861
dc.subjecthemocyanin
dc.subjectimmunoglobulin a
dc.subjectprecipitin
dc.subjectadult
dc.subjectantibody production
dc.subjectantigen antibody reaction
dc.subjectbiological model
dc.subjectclinical article
dc.subjectcolostrum
dc.subjectcontrolled study
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectpriority journal
dc.subjectprozone
dc.subjecttobacco mosaic virus
dc.subjectAnimal
dc.subjectAntigen-Antibody Reactions
dc.subjectCentrifugation, Density Gradient
dc.subjectChickens
dc.subjectColostrum
dc.subjectFemale
dc.subjectHemocyanin
dc.subjectHuman
dc.subjectImmunodiffusion
dc.subjectImmunoelectrophoresis, Two-Dimensional
dc.subjectImmunoglobulin A
dc.subjectImmunoglobulin G
dc.subjectPrecipitin Tests
dc.subjectPregnancy
dc.subjectRabbits
dc.subjectSnails
dc.subjectSupport, Non-U.S. Gov't
dc.subjectTobacco Mosaic Virus
dc.titleSteep gradients of inert substances as supports for precipitin reactions
dc.typeArticle
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