Female fertility and single nucleotide polymorphism comparisons in Cylindrocladium pauciramosum

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dc.contributor.authorSchoch C.L.
dc.contributor.authorCrous P.W.
dc.contributor.authorPolizzi G.
dc.contributor.authorKoike S.T.
dc.date.accessioned2011-05-15T16:02:15Z
dc.date.available2011-05-15T16:02:15Z
dc.date.issued2001
dc.description.abstractCylindrocladium pauciramosum is well established in South America, and has recently been collected from nurseries in South Africa, Italy, and the United States. Isolates were compared with respect to the percentages of hermaphrodites and the respective mating types in the different samples. Based on these data, the effective population size could be determined for the different areas studied. All nurseries had mating type ratios significantly different from an idealized 1:1 ratio. In the South African nursery, the MAT-1 mating type was dominant, while the MAT-2 mating type dominated in other samplings. This is consistent with an introduction of a small starter population. High percentages of hermaphrodites also agreed with recent introductions into nurseries in Italy and the United States. Variability of DNA sequences of the 5′ end of the β-tubulin gene from a set of C. pauciramosum isolates from different geographic regions was low to high. Isolates from South Africa, the United States, and Australia had identical β-tubulin DNA sequences; this sequence was also found in the Italian sample, along with another unique group. Finally, a group of isolates obtained from South and Central America had the highest variation of all isolates investigated, and also included isolates that shared single nucleotide variations with another species, C. candelabrum. These findings suggest that C. pauciramosum most likely has a Central or South American center of origin.
dc.description.versionArticle
dc.identifier.citationPlant Disease
dc.identifier.citation85
dc.identifier.citation9
dc.identifier.issn1912917
dc.identifier.urihttp://hdl.handle.net/10019.1/12385
dc.subjectbeta tubulin
dc.subjectDNA sequence
dc.subjectfemale fertility
dc.subjectgenetic polymorphism
dc.subjectgenetic similarity
dc.subjectgenetic variance
dc.subjecthermaphroditism
dc.subjectItaly
dc.subjectmating system
dc.subjectnursery
dc.subjectSouth Africa
dc.subjectUnited States
dc.subjectDiseases
dc.subjectDNA
dc.subjectGenes
dc.subjectSampling
dc.subjectNurseries
dc.subjectPlants (botany)
dc.subjectCylindrocladium candelabrum
dc.subjectCylindrocladium pauciramosum
dc.titleFemale fertility and single nucleotide polymorphism comparisons in Cylindrocladium pauciramosum
dc.typeArticle
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