A qualitative PCR minipool strategy to screen for virologic failure and antiretroviral drug resistance in South African patients on first-line antiretroviral therapy

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dc.contributor.authorNewman, Howarden_ZA
dc.contributor.authorBreunig, Lukasen_ZA
dc.contributor.authorVan Zyl, Gerten_ZA
dc.contributor.authorStich, Augusten_ZA
dc.contributor.authorPreiser, Wolfgangen_ZA
dc.contributor.otherStellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology: Medical Virology.en_ZA
dc.date.accessioned2015-01-13T11:46:12Z
dc.date.available2015-09-01T03:00:11Z
dc.date.issued2014-08en_ZA
dc.descriptionPlease cite as follows: Newman, N. et al. 2014. A qualitative PCR minipool strategy to screen for virologic failure and antiretroviral drug resistance in South African patients on first-line antiretroviral therapy. Journal of Clinical Virology, 60(4):387-391, doi:/10.1016/j.jcv.2014.05.011.en_ZA
dc.descriptionThe original publication is available at http://www.journalofclinicalvirology.com/article/S1386-6532%2814%2900188-7/pdfen_ZA
dc.descriptionThesis (MMed)--Stellenbosch University, 2014.en_ZA
dc.descriptionCreative Commons Attribution Non-Commercial No Derivatives License 4.0 CC BY-NC-NDen_ZA
dc.description.abstractENGLISH ABSTRACT: Background: The high cost of commercial HIV-1 viral load tests for monitoring of patients on antiretroviral treatment limits their use in resource-constrained settings. Commercial genotypic antiretroviral resistance testing is even more costly, yet it provides important benefits. Objectives: We sought to determine the sensitivity and negative predictive value of a qualitative PCR targeting partial reverse transcriptase for detection of virologic failure when 5 patient specimens are pooled. Study Design: A total of 300 South African routine patient samples were included and tested in 60 pools of 5 samples each. A qualitative nested PCR was optimised for testing pools and individual samples from positive pools. All positive samples were sequenced to detect drug resistance-associated mutations. Results were compared to those of conventional viral load monitoring. Results: Twenty-two of 60 pools tested positive. Individual testing yielded 29 positive individual samples. Twenty-six patients had viral loads of above 1000 copies per millilitre. The pooling algorithm detected 24 of those 26 patients, resulting in a negative predictive value of 99.3%, and a positive predictive value of 89.7%. The sensitivity for detecting patients failing therapy was 92%, with a specificity of 98.9%. Of the patients failing first-line ART, 83.3% had NRTI and 91.7% NNRTI resistance mutations. Conclusions: The pooled testing algorithm presented here required 43% fewer assays than conventional viral load testing. In addition to offering a potential cost saving over individual viral load testing, it also provided drug resistance information which is not available routinely in resourced-limited settings.en_ZA
dc.description.versionPost-printen_ZA
dc.embargo.terms2015-08-31
dc.format.extent23 pagesen_ZA
dc.identifier.citationNewman, N. et al. 2014. A qualitative PCR minipool strategy to screen for virologic failure and antiretroviral drug resistance in South African patients on first-line antiretroviral therapy. Journal of Clinical Virology, 60(4):387-391, doi:/10.1016/j.jcv.2014.05.011.en_ZA
dc.identifier.issn1873-5967 (online)
dc.identifier.issn1386-6532 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/95724
dc.language.isoen_ZAen_ZA
dc.publisherElsevieren_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectSpecimen poolingen_ZA
dc.subjectAntiretroviral agentsen_ZA
dc.subjectHIV (Viruses) -- Treatmenten_ZA
dc.subjectDrug resistanceen_ZA
dc.subjectHIV monitoringen_ZA
dc.titleA qualitative PCR minipool strategy to screen for virologic failure and antiretroviral drug resistance in South African patients on first-line antiretroviral therapyen_ZA
dc.typeArticleen_ZA
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