Dynamics of indigenous bacterial communities associated with crude oil degradation in soil microcosms during nutrient-enhanced bioremediation

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dc.contributor.authorChikere C.B.
dc.contributor.authorSurridge K.
dc.contributor.authorOkpokwasili G.C.
dc.contributor.authorCloete T.E.
dc.date.accessioned2012-04-12T08:33:27Z
dc.date.available2012-04-12T08:33:27Z
dc.date.issued2012
dc.description.abstractBacterial population dynamics were examined during bioremediation of an African soil contaminated with Arabian light crude oil and nutrient enrichment (biostimulation). Polymerase chain reaction followed by denaturing gradient gel electrophoresis (DGGE) were used to generate bacterial community fingerprints of the different treatments employing the 16S ribosomal ribonucleic acid (rRNA) gene as molecular marker. The DGGE patterns of the nutrient-amended soils indicated the presence of distinguishable bands corresponding to the oil-contaminated-nutrient-enriched soils, which were not present in the oil-contaminated and pristine control soils. Further characterization of the dominant DGGE bands after excision, reamplification and sequencing revealed that Corynebacterium spp., Dietzia spp., Rhodococcus erythropolis sp., Nocardioides sp., Low G+C (guanine plus cytosine) Gram positive bacterial clones and several uncultured bacterial clones were the dominant bacterial groups after biostimulation. Prominent Corynebacterium sp. IC10 sequence was detected across all nutrient-amended soils but not in oil-contaminated control soil. Total heterotrophic and hydrocarbon utilizing bacterial counts increased significantly in the nutrient-amended soils 2 weeks post contamination whereas oil-contaminated and pristine control soils remained fairly stable throughout the experimental period. Gas chromatographic analysis of residual hydrocarbons in biostimulated soils showed marked attenuation of contaminants starting from the second to the sixth week after contamination whereas no significant reduction in hydrocarbon peaks were seen in the oil-contaminated control soil throughout the 6-week experimental period. Results obtained indicated that nutrient amendment of oil-contaminated soil selected and enriched the bacterial communities mainly of the Actinobacteria phylogenetic group capable of surviving in toxic contamination with concomitant biodegradation of the hydrocarbons. The present study therefore demonstrated that the soil investigated harbours hydrocarbon-degrading bacterial populations which can be biostimulated to achieve effective bioremediation of oil-contaminated soil. © The Author(s) 2012.
dc.identifier.citationWaste Management and Research
dc.identifier.citation30
dc.identifier.citation3
dc.identifier.citation225
dc.identifier.citation236
dc.identifier.issn0734242X
dc.identifier.other10.1177/0734242X11410114
dc.identifier.urihttp://hdl.handle.net/10019.1/20699
dc.subject16S rRNA
dc.subjectBioremediation
dc.subjectbiostimulation
dc.subjectdenaturing gradient gel electrophoresis
dc.subjecthydrocarbon degradation
dc.subjectpolymerase chain reaction
dc.titleDynamics of indigenous bacterial communities associated with crude oil degradation in soil microcosms during nutrient-enhanced bioremediation
dc.typeReview
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