Sustained glycolytic oscillations in individual isolated yeast cells

Simple item page
dc.contributor.authorGustavsson A.-K.
dc.contributor.authorVan Niekerk D.D.
dc.contributor.authorAdiels C.B.
dc.contributor.authorDu Preez F.B.
dc.contributor.authorGoksor M.
dc.contributor.authorSnoep J.L.
dc.date.accessioned2012-08-17T12:30:49Z
dc.date.available2012-08-17T12:30:49Z
dc.date.issued2012
dc.descriptionArticle
dc.description.abstractYeast glycolytic oscillations have been studied since the 1950s in cell-free extracts and intact cells. For intact cells, sustained oscillations have so far only been observed at the population level, i.e. for synchronized cultures at high biomass concentrations. Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. This is the first time that sustained limit-cycle oscillations have been demonstrated in isolated yeast cells. © 2012 FEBS.
dc.identifier.citationFEBS Journal
dc.identifier.citation279
dc.identifier.citation16
dc.identifier.citation2837
dc.identifier.citation2847
dc.identifier.issn1742464X
dc.identifier.other10.1111/j.1742-4658.2012.08639.x
dc.identifier.urihttp://hdl.handle.net/10019.1/49266
dc.subjectcell heterogeneity
dc.subjectglycolysis
dc.subjectlimit-cycle oscillation
dc.subjectmathematical model
dc.subjectmicrofluidic chamber
dc.titleSustained glycolytic oscillations in individual isolated yeast cells
Files
Collections
Stellenbosch University - Scopus Publications