Glucose tolerance, MTHFR C677T and NOS3 G894T polymorphisms, and global DNA methylation in mixed ancestry African individuals

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dc.contributor.authorMatsha, Tandi E.en_ZA
dc.contributor.authorPheiffer, Carmenen_ZA
dc.contributor.authorMutize, Tinasheen_ZA
dc.contributor.authorErasmus, Rajiv T.en_ZA
dc.contributor.authorKengne, Andre P.en_ZA
dc.date.accessioned2017-06-12T08:04:50Z
dc.date.available2017-06-12T08:04:50Z
dc.date.issued2016-08-14
dc.descriptionCITATION: Matsha, T. E. et al. 2016. Glucose tolerance, MTHFR C677T and NOS3 G894T polymorphisms, and global DNA methylation in mixed ancestry African individuals. Journal of Diabetes Research, Article ID 8738072, doi:10.1155/2016/8738072.
dc.descriptionThe original publication is available at https://www.hindawi.com/journals/jdr
dc.description.abstractENGLISH ABSTRACT: The aim of this study is to quantify global DNA methylation and investigate the relationship with diabetes status and polymorphisms in MTHFR C677T and NOS3 G894T genes in mixed ancestry subjects from South Africa. Global DNA methylation was measured, and MTHFR rs1801133 and NOS3 rs1799983 polymorphisms were genotyped using high throughput real-time polymerase chain reaction and direct DNA sequencing. Of the 564 participants, 158 (28%) individuals had T2DM of which 97 (17.2%) were screen-detected cases. Another 119 (21.1%) had prediabetes, that is, impaired fasting glucose, impaired glucose tolerance, or the combination of both, and the remainder 287 (50.9%) had normal glucose tolerance. Global DNA methylation was significantly higher in prediabetes and screen-detected diabetes than in normal glucose tolerance (both ) and in screen-detected diabetes compared to known diabetes on treatment (). There was no difference in global DNA methylation between known diabetes on treatment and normal glucose tolerance (). In multivariable linear regression analysis, only NOS3 was associated with increasing global DNA methylation (; 95% CI: 0.286 to 1.560). The association of global DNA methylation with screen-detected diabetes but not treated diabetes suggests that glucose control agents to some extent may be reversing DNA methylation. The association between NOS3 rs1799983 polymorphisms and DNA methylation suggests gene-epigenetic mechanisms through which vascular diabetes complications develop despite adequate metabolic control.en_ZA
dc.description.urihttps://www.hindawi.com/journals/jdr/2016/8738072/
dc.description.versionPublisher's version
dc.format.extent9 pagesen_ZA
dc.identifier.citationMatsha, T. E. et al. 2016. Glucose tolerance, MTHFR C677T and NOS3 G894T polymorphisms, and global DNA methylation in mixed ancestry African individuals. Journal of Diabetes Research, Article ID 8738072, doi:10.1155/2016/8738072.
dc.identifier.issn2314-6753 (online)
dc.identifier.issn2314-6745 (print)
dc.identifier.otherdoi:10.1155/2016/8738072
dc.identifier.urihttp://hdl.handle.net/10019.1/101747
dc.language.isoen_ZAen_ZA
dc.publisherHindawien_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectGlucose tolerance testen_ZA
dc.subjectDiabetes -- Diagnosisen_ZA
dc.subjectInsulin -- Methylationen_ZA
dc.subjectPolymorphisms, Geneticen_ZA
dc.titleGlucose tolerance, MTHFR C677T and NOS3 G894T polymorphisms, and global DNA methylation in mixed ancestry African individualsen_ZA
dc.typeArticleen_ZA
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