African 1, an epidemiologically important clonal complex of mycobacterium bovis dominant in Mali, Nigeria, Cameroon, and Chad

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dc.contributor.authorMuller B.
dc.contributor.authorHilty M.
dc.contributor.authorBerg S.
dc.contributor.authorGarcia-Pelayo M.C.
dc.contributor.authorDale J.
dc.contributor.authorBoschiroli M.L.
dc.contributor.authorCadmus S.
dc.contributor.authorNgandolo B.N.R.
dc.contributor.authorGodreuil S.
dc.contributor.authorDiguimbaye-Djaibe C.
dc.contributor.authorKazwala R.
dc.contributor.authorBonfoh B.
dc.contributor.authorNjanpop-Lafourcade B.M.
dc.contributor.authorSahraoui N.
dc.contributor.authorGuetarni D.
dc.contributor.authorAseffa A.
dc.contributor.authorMekonnen M.H.
dc.contributor.authorRazanamparany V.R.
dc.contributor.authorRamarokoto H.
dc.contributor.authorDjonne B.
dc.contributor.authorOloya J.
dc.contributor.authorMachado A.
dc.contributor.authorMucavele C.
dc.contributor.authorSkjerve E.
dc.contributor.authorPortaels F.
dc.contributor.authorRigouts L.
dc.contributor.authorMichel A.
dc.contributor.authorMuller A.
dc.contributor.authorKallenius G.
dc.contributor.authorVan Helden P.D.
dc.contributor.authorHewinson R.G.
dc.contributor.authorZinsstag J.
dc.contributor.authorGordon S.V.
dc.contributor.authorSmith N.H.
dc.date.accessioned2011-05-15T16:01:52Z
dc.date.available2011-05-15T16:01:52Z
dc.date.issued2009
dc.description.abstractWe have identified a clonal complex of Mycobacterium bovis present at high frequency in cattle in population samples from several sub-Saharan west-central African countries. This closely related group of bacteria is defined by a specific chromosomal deletion (RDAfl) and can be identified by the absence of spacer 30 in the standard spoligotype typing scheme. We have named this group of strains the African 1 (Afl) clonal complex and have defined the spoligotype signature of this clonal complex as being the same as the M. bovis BCG vaccine strain but with the deletion of spacer 30. Strains of the Afl clonal complex were found at high frequency in population samples of M. bovis from cattle in Mali, Cameroon, Nigeria, and Chad, and using a combination of variable-number tandem repeat typing and spoligotyping, we show that the population of M. bovis in each of these countries is distinct, suggesting that the recent mixing of strains between countries is not common in this area of Africa. Strains with the Afl-specific deletion (RDAfl) were not identified in M. bovis isolates from Algeria, Burundi, Ethiopia, Madagascar, Mozambique, South Africa, Tanzania, and Uganda. Furthermore, the spoligotype signature of the Afl clonal complex has not been identified in population samples of bovine tuberculosis from Europe, Iran, and South America. These observations suggest that the Afl clonal complex is geographically localized, albeit to several African countries, and we suggest that the dominance of the clonal complex in this region is the result of an original introduction into cows naïve to bovine tuberculosis. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationJournal of Bacteriology
dc.identifier.citation191
dc.identifier.citation6
dc.identifier.issn219193
dc.identifier.other10.1128/JB.01590-08
dc.identifier.urihttp://hdl.handle.net/10019.1/12192
dc.subjectBCG vaccine
dc.subjectspacer DNA
dc.subjectAlgeria
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectbovine tuberculosis
dc.subjectBurundi
dc.subjectCameroon
dc.subjectChad
dc.subjectchromosome deletion
dc.subjectEthiopia
dc.subjectEurope
dc.subjectgene deletion
dc.subjectIran
dc.subjectMadagascar
dc.subjectMali
dc.subjectMozambique
dc.subjectMycobacterium bovis
dc.subjectNigeria
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpriority journal
dc.subjectSouth Africa
dc.subjectSouth America
dc.subjectTanzania
dc.subjectUganda
dc.subjectvariable number of tandem repeat
dc.subjectAfrica
dc.subjectanimal
dc.subjectbacterium identification
dc.subjectcattle
dc.subjectclassification
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmicrobiology
dc.subjectmolecular genetics
dc.subjectBos
dc.subjectBovinae
dc.subjectMycobacterium bovis
dc.subjectAfrica
dc.subjectAnimals
dc.subjectBacterial Typing Techniques
dc.subjectCattle
dc.subjectChromosome Deletion
dc.subjectMolecular Sequence Data
dc.subjectMycobacterium bovis
dc.subjectTuberculosis, Bovine
dc.titleAfrican 1, an epidemiologically important clonal complex of mycobacterium bovis dominant in Mali, Nigeria, Cameroon, and Chad
dc.typeArticle
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