Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA of Lactobacillus plantarum 423, monitored with real-time PCR

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dc.contributor.authorRamiah K.
dc.contributor.authorvan Reenen C.A.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:56:47Z
dc.date.available2011-05-15T15:56:47Z
dc.date.issued2007
dc.description.abstractExpression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA by Lactobacillus plantarum 423, grown in the presence of bile, pancreatin and at low pH, was studied by real-time PCR. Mub, MapA and EF-Tu were up-regulated in the presence of mucus, proportional to increasing concentrations. Expression of MapA was up-regulated in the presence of 3.0 g/l bile and 3.0 g/l pancreatin at pH 6.5. Similar results were recorded in the presence of 10.0 g/l bile and 10.0 g/l pancreatin at pH 6.5. Expression of Mub was down-regulated in the presence of bile and pancreatin, whilst the expression of EF-Tu and plaA remained unchanged. Expression of Mub and MapA remained unchanged at pH 4.0, whilst expression of EF-Tu and plaA were up-regulated. Expression of MapA was down-regulated in the presence of 1.0 g/l l-cysteine HCl, suggesting that the gene is regulated by transcription attenuation that involves cysteine. © 2007 Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Food Microbiology
dc.identifier.citation116
dc.identifier.citation3
dc.identifier.issn1681605
dc.identifier.other10.1016/j.ijfoodmicro.2007.02.011
dc.identifier.urihttp://hdl.handle.net/10019.1/10041
dc.subjectbacteriocin
dc.subjectcysteine
dc.subjecthydrochloric acid
dc.subjectpancreatin
dc.subjectarticle
dc.subjectbile
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectgene control
dc.subjectgene expression
dc.subjectgenetic transcription
dc.subjectLactobacillus plantarum
dc.subjectmucus
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpH
dc.subjectreal time polymerase chain reaction
dc.subjectupregulation
dc.subjectBacterial Adhesion
dc.subjectBacterial Proteins
dc.subjectBile
dc.subjectGene Expression Regulation, Bacterial
dc.subjectHumans
dc.subjectHydrogen-Ion Concentration
dc.subjectLactobacillus plantarum
dc.subjectMucus
dc.subjectPancreatin
dc.subjectPolymerase Chain Reaction
dc.subjectProbiotics
dc.subjectLactobacillus plantarum
dc.titleExpression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA of Lactobacillus plantarum 423, monitored with real-time PCR
dc.typeArticle
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