Degradation of proteins and starch by combined immobilization of protease, α-amylase and β-galactosidase on a single electrospun nanofibrous membrane
Date
2019-01-31
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease),
were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber
mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes
on their own as well as in different combinations with an additional enzyme, β-galactosidase, on the
same non-woven nanofiber mat. This experiment yielded a universal method for immobilizing
different combinations of enzymes with nanofibrous mats containing maleic anhydride (MAnh)
residues in the polymer backbone.
Description
CITATION: Cloete W. J. et al. 2019. Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane. Molecules, 24(3):508. doi:10.3390/molecules2403050
The original publication is available at https://www.mdpi.com/journal/molecules
The original publication is available at https://www.mdpi.com/journal/molecules
Keywords
Poly(styrene-co-maleic anhydride), Enzymes -- Biotechnology, Immobilization, Protease, Proteolytic enzymes, Immobilized enzymes -- Biotechnology, Proteins -- Degradation, Starch -- Synthesis
Citation
Cloete W. J. et al. 2019. Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane. Molecules, 24(3):508. doi:10.3390/molecules2403050