Medroxyprogesterone acetate alters mycobacterium bovis BCG-induced cytokine production in peripheral blood mononuclear cells of contraceptive users

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dc.contributor.authorKleynhans, Leanieen_ZA
dc.contributor.authorDu Plessis, Nelitaen_ZA
dc.contributor.authorBlack, Gillian F.en_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorKidd, Martinen_ZA
dc.contributor.authorVan Helden, Paul D.
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorRonacher, Katharinaen_ZA
dc.date.accessioned2011-10-13T16:59:03Z
dc.date.available2011-10-13T16:59:03Z
dc.date.issued2011-09-08
dc.descriptionCITATION: Kleynhans, L. et al. 2011. Medroxyprogesterone acetate alters mycobacterium bovis BCG-induced cytokine production in peripheral blood mononuclear cells of contraceptive users. PLoS ONE, 6(9): e24639, doi:10.1371/journal.pone.0024639.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractMost individuals latently infected with Mycobacterium tuberculosis (M.tb) contain the infection by a balance of effector and regulatory immune responses. This balance can be influenced by steroid hormones such as glucocorticoids. The widely used contraceptive medroxyprogesterone acetate (MPA) possesses glucocorticoid activity. We investigated the effect of this hormone on immune responses to BCG in household contacts of active TB patients. Multiplex bead array analysis revealed that MPA demonstrated both glucocorticoid and progestogenic properties at saturating and pharmacological concentrations in peripheral blood mononuclear cells (PBMCs) and suppressed antigen specific cytokine production. Furthermore we showed that PBMCs from women using MPA produced significantly lower levels of IL-1α, IL-12p40, IL-10, IL-13 and G-CSF in response to BCG which corresponded with lower numbers of circulating monocytes observed in these women. Our research study is the first to show that MPA impacts on infections outside the genital tract due to a systemic effect on immune function. Therefore MPA use could alter susceptibility to TB, TB disease severity as well as change the efficacy of new BCG-based vaccines, especially prime-boost vaccine strategies which may be administered to adult or adolescent women in the future. © 2011 Kleynhans et al.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0024639
dc.description.versionPublisher's version
dc.format.extent10 pages
dc.identifier.citationKleynhans, L. et al. 2011. Medroxyprogesterone acetate alters mycobacterium bovis BCG-induced cytokine production in peripheral blood mononuclear cells of contraceptive users. PLoS ONE, 6(9): e24639, doi:10.1371/journal.pone.0024639.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0024639
dc.identifier.urihttp://hdl.handle.net/10019.1/16961
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectTuberculosisen_ZA
dc.subjectMedroxyprogesterone acetateen_ZA
dc.subjectBCG vaccinesen_ZA
dc.titleMedroxyprogesterone acetate alters mycobacterium bovis BCG-induced cytokine production in peripheral blood mononuclear cells of contraceptive usersen_ZA
dc.typeArticle
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