'n Vergelykende studie tussen tweeselembrio's van CBA- en F1-muise in 'n menslike in vitro bevrugtingsprogram

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dc.contributor.authorKruger, T. F.
dc.contributor.authorStander, F. S. H.
dc.date.accessioned2011-03-18T14:57:40Z
dc.date.available2011-03-18T14:57:40Z
dc.date.issued1984-02
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.description.abstractThe two-cell mouse embryo can develop in a chemically defined medium to the blastocyst stage but conditions must be strictly controlled. Fresh T6 (Whittingham's) medium is being used weekly for this purpose in our laboratory and subjected to a 5% CO2-in-air system 24 hours prior to use. Experiments were designed to determine whether two-cell embryos obtained from an F1 (CBA x C57/B1/6) x F1 cross were superior to the easily obtainable CBA x ICR cross. The aim was to get a 90% blastocyst result for use as a quality control. Eight experiments were performed over a 6-week period. The CBA two-cell embryos were compared with the F1 group under identical conditions. A total of 177 two-cell embryos were obtained from the CBA group and 214 two-cell embryos from the F1 group. In the CBA group 51 developed to the blastocyst stage (28.8%) compared with 187 in the F1 group (87,4%) (P<0.0001). In conclusion, it is advisable to obtain F1 mice to use weekly in the laboratory as a quality control measure. The CBA group yields poor cultures, and this lead to unnecessary laboratory confusion.en_ZA
dc.description.versionPublishers' Version
dc.format.extent2 p.
dc.identifier.citationKruger, T. F., & Stander, F. S. H. 1984. 'n Vergelykende studie tussen tweeselembrio's van CBA- en F1-muise in 'n menslike in vitro bevrugtingsprogram. South African Medical Journal, 65, 209-210.
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/7567
dc.language.isoaf_ZA
dc.publisherHealth and Medical Publishing Group (HMPG)
dc.rights.holderSAMJ reserves copyright of the material published
dc.subjectIn vitro fertilizationen_ZA
dc.title'n Vergelykende studie tussen tweeselembrio's van CBA- en F1-muise in 'n menslike in vitro bevrugtingsprogramaf_ZA
dc.title.alternativeA comparative study of two-cell embryos of CBA and F1 mice in a human in vitro fertilization programmeen_ZA
dc.typeArticle
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