High performance liquid chromatography analysis of wine anthocyanins revisited: Effect of particle size and temperature
Date
2009
Authors
de Villiers A.
Cabooter D.
Lynen F.
Desmet G.
Sandra P.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The complex anthocyanin fraction of red wines poses a demanding analytical challenge. We have found that anthocyanins are characterised by extremely low optimal chromatographic velocities, and as a consequence generic HPLC methods suffer from limited resolving power. Slow on-column inter-conversion reactions, particularly between carbinol and flavylium species, are shown to occur on the same time scale as chromatographic separation, leading to increased plate heights at normal chromatographic velocities. In order to improve current routine HPLC separations, the use of small (1.7 μm) particles and high temperature liquid chromatography (HTLC) were investigated. 1.7 μm particles provide better efficiency and higher optimal linear velocities, although column lengths of ∼20 cm should be used to avoid the detrimental effects of conversion reactions. More importantly, operation at temperatures up to 50 °C increases the kinetics of inter-conversion reactions, and implies significantly improved efficiency under relatively mild analysis conditions. It is further demonstrated using relevant kinetic data that no on-column thermal degradation of these thermally labile compounds is observed at 50 °C and analysis times of <2 h. © 2009 Elsevier B.V. All rights reserved.
Description
Keywords
Anthocyanins, Elevated temperature, HPLC, Small particles, Thermal degradation, Body fluids, Chromatographic analysis, Chromatography, Degradation, High pressure liquid chromatography, Liquids, Methanol, Particle size analysis, Thermal effects, Thermogravimetric analysis, Wine, High performance liquid chromatography, anthocyanin, methanol, article, chemical analysis, food analysis, high performance liquid chromatography, high temperature, particle size, priority journal, wine, Anthocyanins, Chromatography, High Pressure Liquid, Drug Stability, Hydrogen-Ion Concentration, Kinetics, Particle Size, Plant Extracts, Temperature, Wine
Citation
Journal of Chromatography A
1216
15
1216
15