Purification, partial amino acid sequence and mode of action of pediocin PD-1, a bacteriocin produced by Pediococcus damnosus NCFB 1832.

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dc.contributor.authorBauer R.
dc.contributor.authorChikindas M.L.
dc.contributor.authorDicks L.M.
dc.date.accessioned2011-05-15T15:56:48Z
dc.date.available2011-05-15T15:56:48Z
dc.date.issued2005
dc.description.abstractPediocin PD-1 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus damnosus NCFB1832. It inhibits the growth of several food spoilage bacteria, including malolactic bacteria isolated from wine. Pediocin PD-1 is 2866.87+/-0.4 Da in size, has an isoelectric point (pI) of ca. 9.0 and, on amino acid composition, has partial homology to the lantibiotic plantaricin C. The highest activity of pediocin PD-1 against cells of Oenococcus oeni was observed at an external pH of 5.0 and at 25 degrees C. The primary mode of action of pediocin PD-1 is most probably due to pore formation, as indicated by the efflux of K+ from metabolically active cells of O. oeni. In the presence of 10 mM gadolinium (Gd3+), pediocin PD-1 did not affect cells of O. oeni. This suggests that the mode of action of pediocin PD-1 relies on a net negatively charged cell surface. In comparison to nisin, pediocin PD-1 is less active against non-growing cells of O. oeni.
dc.description.versionArticle
dc.identifier.citationInternational journal of food microbiology
dc.identifier.citation101
dc.identifier.citation1
dc.identifier.issn1681605
dc.identifier.urihttp://hdl.handle.net/10019.1/10049
dc.subjectbacteriocin
dc.subjectpediocin PD 1
dc.subjectpediocin PD-1
dc.subjectamino acid sequence
dc.subjectarticle
dc.subjectchemistry
dc.subjectfood contamination
dc.subjectfood control
dc.subjectisoelectric point
dc.subjectisolation and purification
dc.subjectmass spectrometry
dc.subjectmetabolism
dc.subjectmicrobiological examination
dc.subjectmicrobiology
dc.subjectmolecular weight
dc.subjectPediococcus
dc.subjectpH
dc.subjectsequence alignment
dc.subjectsequence homology
dc.subjecttemperature
dc.subjectwine
dc.subjectAmino Acid Sequence
dc.subjectBacteriocins
dc.subjectFood Contamination
dc.subjectFood Microbiology
dc.subjectHydrogen-Ion Concentration
dc.subjectIsoelectric Point
dc.subjectMass Spectrometry
dc.subjectMicrobial Sensitivity Tests
dc.subjectMolecular Weight
dc.subjectPediococcus
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectTemperature
dc.subjectWine
dc.titlePurification, partial amino acid sequence and mode of action of pediocin PD-1, a bacteriocin produced by Pediococcus damnosus NCFB 1832.
dc.typeArticle
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