Modeling the minimum enzymatic requirements for optimal cellulose conversion

Simple item page
dc.contributor.authorDen Haan, R.
dc.contributor.authorVan Zyl, J. M.
dc.contributor.authorHarms, T. M.
dc.contributor.authorVan Zyl, W. H.
dc.date.accessioned2013-07-29T06:21:43Z
dc.date.available2013-07-29T06:21:43Z
dc.date.issued2013-04
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.descriptionThe original publication is available at http://iopscience.iop.org/1748-9326/en_ZA
dc.description.abstractHydrolysis of cellulose is achieved by the synergistic action of endoglucanases, exoglucanases and -glucosidases. Most cellulolytic microorganisms produce a varied array of these enzymes and the relative roles of the components are not easily defined or quantified. In this study we have used partially purified cellulases produced heterologously in the yeast Saccharomyces cerevisiae to increase our understanding of the roles of some of these components. CBH1 (Cel7), CBH2 (Cel6) and EG2 (Cel5) were separately produced in recombinant yeast strains, allowing their isolation free of any contaminating cellulolytic activity. Binary and ternary mixtures of the enzymes at loadings ranging between 3 and 100 mg g-1 Avicel allowed us to illustrate the relative roles of the enzymes and their levels of synergy. A mathematical model was created to simulate the interactions of these enzymes on crystalline cellulose, under both isolated and synergistic conditions. Laboratory results from the various mixtures at a range of loadings of recombinant enzymes allowed refinement of the mathematical model. The model can further be used to predict the optimal synergistic mixes of the enzymes. This information can subsequently be applied to help to determine the minimum protein requirement for complete hydrolysis of cellulose. Such knowledge will be greatly informative for the design of better enzymatic cocktails or processing organisms for the conversion of cellulosic biomass to commodity products.en_ZA
dc.description.sponsorshipStellenbosch Universityen_ZA
dc.description.versionPublishers' versionen_ZA
dc.format.extent11 p. : ill.
dc.identifier.citationDen Haan, R., Van Zyl, J. M., Harms, T. M. & Van Zyl, W. H. 2013. Modeling the minimum enzymatic requirements for optimal cellulose conversion. Environmental Research Letters, 8(2), doi:10.1088/1748-9326/8/2/025013.en_ZA
dc.identifier.issn1748-9326 (print)
dc.identifier.issn1748-9326 (online)
dc.identifier.otherdoi:10.1088/1748-9326/8/2/025013
dc.identifier.urihttp://hdl.handle.net/10019.1/85258
dc.language.isoen_ZAen_ZA
dc.publisherIoP Publisingen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectCellulose -- Hydrolysis ofen_ZA
dc.subjectCellulose conversionen_ZA
dc.subjectConsolidated bioprocessingen_ZA
dc.subjectCellobiohydrolaseen_ZA
dc.subjectEndoglucanaseen_ZA
dc.titleModeling the minimum enzymatic requirements for optimal cellulose conversionen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
denhaan_modeling_2013.pdf
Size:
656.9 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.95 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Microbiology)