Construction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSD

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dc.contributor.advisorSwart, Pieteren_ZA
dc.contributor.advisorSnoep, Jacob Leendert en_ZA
dc.contributor.authorVan Schalkwyk, Frederick Josephus (Erick)en_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Department of Biochemistryen_ZA
dc.date.accessioned2016-03-09T14:54:15Z
dc.date.available2016-03-09T14:54:15Z
dc.date.issued2016-03
dc.description.abstractENGLISH ABSTRACT : This study describes: how time-dependent experimental data, describing the reactions catalyzed by ovine cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17), was obtained using pregnenolone and progesterone as substrates ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine CYP17 ; how the time-dependent metabolism of pregnenolone, 17α-hydroxypregnenolone and dehydroepiandrosterone by ovine 3β-hydroxysteroid dehydrogenase (3β-HSD), was obtained ; the construction and validation of a kinetic model and determining the Km and Vmax - values for each reaction catalyzed by ovine 3β-HSDen_ZA
dc.description.abstractAFRIKAANSE OPSOMMING : Hierdie studie beskryf: hoe tyd-afhanklike eksperimentele data van die reaksies wat gekataliseer word deur skaap sitochroom P450 17α-hidroksielase/17,20-liase (CYP17) met pregnenoloon en progesteroon as substraat, verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap CYP17 gekataliseer word ; hoe die tyd-afhanklike metabolisme van pregnenoloon, progesteroon en dehidroepiandrosteroon wat gekataliseer word deur skaap 3β-hidroksiesteroïd dehidroginase (3β-HSD), verkry is ; die konstruksie en validasie van die kinetiese model asook die bepaling van die Km - en Vmax - waardes vir elke reaksie wat deur skaap 3β-HSD gekataliseer wordaf_ZA
dc.description.uriThesis (MSc)--Stellenbosch University, 2016en_ZA
dc.format.extentxiii, 125 pages : illustrations (chiefly colour)en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/98727
dc.language.isoen_ZAen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.rights.holderStellenbosch Universityen_ZA
dc.subjectSteroid hormonesen_ZA
dc.subjectBiosynthesis -- Modelsen_ZA
dc.subjectSteroidogenesis -- Modelsen_ZA
dc.subjectAdrenocortical hormones -- Modelsen_ZA
dc.subjectKidneys -- Physiologyen_ZA
dc.subjectSheep -- Physiologyen_ZA
dc.subjectUCTDen_ZA
dc.titleConstruction and analysis of a kinetic model of the ovine adrenal steroidogenic subsystem comprising of CYP17 and 3β-HSDen_ZA
dc.typeThesisen_ZA
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