Production of bacteriocin ST33LD, produced by Leuconostoc mesenteroides subsp. mesenteroides, as recorded in the presence of different medium components

Simple item page
dc.contributor.authorTodorov S.D.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T16:05:32Z
dc.date.available2011-05-15T16:05:32Z
dc.date.issued2005
dc.description.abstractBacteriocin ST33LD, produced by Leuconostoc mesenteroides subsp. mesenteroides, is approximately 2.7 kDa in size and inhibits Enterococcus faecalis, Escherichia coli, Lactobacillus casei and Pseudomonas aeruginosa. Good growth was recorded in the presence of 10% (w/v) soy milk or 10% (w/v) molasses, but there was no bacteriocin production. Growth in MRS broth adjusted to pH 4.5 yielded low bacteriocin levels (800 AU/ml). However, the same medium adjusted to pH 5.0, 5.5 and 6.5, respectively, yielded 3200 AU/ml. Tween 80 decreased bacteriocin production by more than 50%. Growth in the presence of tryptone yielded maximal activity (12,800 AU/ml), whereas different combinations of tryptone, meat extract and yeast extract produced activity levels of 1600 AU/ml and less. Growth in the presence of 2.0% (w/v) sucrose, or maltose, yielded much higher levels of bacteriocin activity (12,800 AU/ml) compared to growth in the presence of 2.0% (w/v) glucose or lactose (6400 AU/ml). Lower yields were also recorded in the presence of fructose and mannose. KH 2PO4 at 10.0% (w/v) stimulated bacteriocin production. Glycerol concentrations of 0.5% (w/v) and higher (up to 5.0%, w/v) repressed bacteriocin production by 50%. The addition of cyanocobalamin, thiamine and L-ascorbic acid to MRS broth (1.0 ppm) yielded 12,800 AU/ml bacteriocin, whereas the addition of DL-6,8-thioctic acid yielded only 6 400 AU/ml. © Springer 2005.
dc.description.versionArticle
dc.identifier.citationWorld Journal of Microbiology and Biotechnology
dc.identifier.citation21
dc.identifier.citation09-Aug
dc.identifier.issn9593993
dc.identifier.other10.1007/s11274-005-8122-4
dc.identifier.urihttp://hdl.handle.net/10019.1/13178
dc.subjectEscherichia coli
dc.subjectGrowth kinetics
dc.subjectOrganic acids
dc.subjectpH effects
dc.subjectYeast
dc.subjectBacteriocin ST33LD
dc.subjectLeuconostoc mesenteroides
dc.subjectOlive brine
dc.subjectBacteria
dc.subjectEnterococcus faecalis
dc.subjectEscherichia coli
dc.subjectGlycine max
dc.subjectLactobacillus casei
dc.subjectLeuconostoc mesenteroides
dc.subjectLeuconostoc mesenteroides subsp. mesenteroides
dc.subjectPseudomonas aeruginosa
dc.titleProduction of bacteriocin ST33LD, produced by Leuconostoc mesenteroides subsp. mesenteroides, as recorded in the presence of different medium components
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications