From steady-state to synchronized yeast glycolytic oscillations II: Model validation

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dc.contributor.authorDu Preez F.B.
dc.contributor.authorVan Niekerk D.D.
dc.contributor.authorSnoep J.L.
dc.date.accessioned2012-08-17T12:32:19Z
dc.date.available2012-08-17T12:32:19Z
dc.date.issued2012
dc.descriptionArticle
dc.description.abstractIn an accompanying paper [du Preez et al., (2012) FEBS J279, 2810-2822], we adapt an existing kinetic model for steady-state yeast glycolysis to simulate limit-cycle oscillations. Here we validate the model by testing its capacity to simulate a wide range of experiments on dynamics of yeast glycolysis. In addition to its description of the oscillations of glycolytic intermediates in intact cells and the rapid synchronization observed when mixing out-of-phase oscillatory cell populations (see accompanying paper), the model was able to predict the Hopf bifurcation diagram with glucose as the bifurcation parameter (and one of the bifurcation points with cyanide as the bifurcation parameter), the glucose- and acetaldehyde-driven forced oscillations, glucose and acetaldehyde quenching, and cell-free extract oscillations (including complex oscillations and mixed-mode oscillations). Thus, the model was compliant, at least qualitatively, with the majority of available experimental data for glycolytic oscillations in yeast. To our knowledge, this is the first time that a model for yeast glycolysis has been tested against such a wide variety of independent data sets. © 2012 FEBS.
dc.identifier.citationFEBS Journal
dc.identifier.citation279
dc.identifier.citation16
dc.identifier.citation2823
dc.identifier.citation2836
dc.identifier.issn1742464X
dc.identifier.other10.1111/j.1742-4658.2012.08658.x
dc.identifier.urihttp://hdl.handle.net/10019.1/49269
dc.subjectglycolysis
dc.subjectlimit-cycle oscillation
dc.subjectmathematical model
dc.subjectmodel validation
dc.subjectSaccharomyces cerevisiae
dc.titleFrom steady-state to synchronized yeast glycolytic oscillations II: Model validation
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