An ffh mutant of Streptococcus mutans is viable and able to physiologically adapt to low pH in continuous culture

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dc.contributor.authorCrowley P.J.
dc.contributor.authorSvensater G.
dc.contributor.authorSnoep J.L.
dc.contributor.authorBleiweis A.S.
dc.contributor.authorBrady L.J.
dc.date.accessioned2011-05-15T16:04:57Z
dc.date.available2011-05-15T16:04:57Z
dc.date.issued2004
dc.description.abstractPreviously, we described in Streptococcus mutans strain NG8 a 5-gene operon (sat) that includes ffh, the bacterial homologue of the eukaryotic signal recognition particle (SRP) protein, SR54. A mutation in ffh resulted in acid sensitivity but not loss of viability. In the present study, chemostat-grown cells of the ffh mutant were shown to possess only 26% and 39% of the parental membrane F-ATPase activity and 55% and 75% of parental glucose- phosphotransferase (PTS) activity when pH-7 and pH-5-grown cells, respectively, were assayed. Two-dimensional-gel electrophoretic analyses revealed significant differences in protein profiles between parent and ffh-mutant strains at both pH 5 and pH 7. It appears that the loss of active SRP (Ffh) function, while not lethal, results in substantial alterations in cellular physiology that includes acid tolerance. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationFEMS Microbiology Letters
dc.identifier.citation234
dc.identifier.citation2
dc.identifier.issn3781097
dc.identifier.other10.1016/j.femsle.2004.03.043
dc.identifier.urihttp://hdl.handle.net/10019.1/12899
dc.subjectadenosine triphosphatase
dc.subjectglucokinase
dc.subjectsignal recognition particle
dc.subjectacid tolerance
dc.subjectacidity
dc.subjectalkalinity
dc.subjectarticle
dc.subjectbacterial cell
dc.subjectbacterial gene
dc.subjectbacterial growth
dc.subjectbacterial survival
dc.subjectbacterium culture
dc.subjectbacterium mutant
dc.subjectcell viability
dc.subjectchemostat
dc.subjectcontinuous culture
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein function
dc.subjectStreptococcus mutans
dc.subjecttwo dimensional gel electrophoresis
dc.subjectAcclimatization
dc.subjectBacterial Proteins
dc.subjectCell Membrane
dc.subjectHydrogen-Ion Concentration
dc.subjectKinetics
dc.subjectOperon
dc.subjectPhosphoenolpyruvate Sugar Phosphotransferase System
dc.subjectProton-Translocating ATPases
dc.subjectSignal Recognition Particle
dc.subjectStreptococcus mutans
dc.subjectSubstrate Specificity
dc.subjectBacteria (microorganisms)
dc.subjectEukaryota
dc.subjectStreptococcus
dc.subjectStreptococcus mutans
dc.titleAn ffh mutant of Streptococcus mutans is viable and able to physiologically adapt to low pH in continuous culture
dc.typeArticle
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