Near-isogenic lines of triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during diuraphis noxia feeding
Date
2014
Authors
Botha, Anna-Maria
Van Eck, Leon
Burger, N. Francois V.
Swanevelder, Zacharias H.
Journal Title
Journal ISSN
Volume Title
Publisher
The Company of Biologists Ltd
Abstract
Russian wheat aphid (Diuraphis noxia, Kurdjumov) feeding on
susceptible Triticum aestivum L. leads to leaf rolling, chlorosis and
plant death – symptoms not present in resistant lines. Although the
effects of several D. noxia (Dn) resistance genes are known, none
have been isolated or characterized. Wheat varieties expressing
different Dn genes exhibit distinct modes of D. noxia resistance, such
as antibiosis (Dn1), tolerance (Dn2), and antixenosis (Dn5). However,
the mechanism whereby feeding aphids are perceived, and how
subsequent transcriptional responses are partitioned into resistance
categories, remains unclear. Here we report on downstream events in
near-isogenic wheat lines containing different Dn genes after D. noxia
biotype SA1 feeding. Transcripts involved in stress, signal transduction,
photosynthesis, metabolism and gene regulation were differentially
regulated during D. noxia feeding. Expression analyses using RTqPCR
and RNA hybridization, as well as enzyme activity profiling,
provide evidence that the timing and intensity of pathways induced are
critical in the development of particular modes of resistance. Pathways
involved include the generation of kinase signalling cascades that lead
to a sustained oxidative burst, and a hypersensitive response that is
active during antibiosis. Tolerance is a passive resistance mechanism
that acts through repair or de novo synthesis of photosystem proteins.
Results further suggest that ethylene-mediated pathways are possibly
involved in generating volatile compounds and cell wall fortification
during the antixenosic response.
Description
CITATION: Botha, A-M., Van Eck, L., Burger, N. F. V. & Swanevelder, Z. H. 2014. Near-isogenic lines of Triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during Diuraphis noxia feeding. Biology Open, 31116-1126, doi:10.1242/bio.201410280.
The original publication is available at http://bio.biologists.org/content/early/2014/10/17/bio.201410280
The original publication is available at http://bio.biologists.org/content/early/2014/10/17/bio.201410280
Keywords
Citation
Botha, A-M., Van Eck, L., Burger, N. F. V. & Swanevelder, Z. H. 2014. Near-isogenic lines of Triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during Diuraphis noxia feeding. Biology Open, 3:1116-1126, doi:10.1242/bio.201410280.