Stable transformation of Hi-II maize using the particle inflow gun
Embryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, was transformed by microprojectile bombardment using a particle inflow gun. This highly regenerable maize line was transformed with the plasmid pAHC25, a dual expression vector containing the uidA reporter gene and the bar selectable marker gene, both under the control of the maize ubiquitin promoter. Transgenic plants were obtained from 10 independent transformation events. Transmission of the bar and uidA genes to the progeny of three of these lines was demonstrated by Southern blot analysis and by transgene expression.