A broad host range reporter plasmid for the analysis of divergent promoter regions
Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
Academy of Science of South Africa
Abstract
Although many vectors exist for Escherichia coli and closely related species, there are few broad host range vectors that can be conjugated into a large variety of Gram-negative bacteria. We have constructed a broad host range vector, pMJ445, that facilitates the analysis of divergent promoters in Gram-negative bacteria. The vector was validated using two intergenic regions derived from gene clusters involved in hydantoin hydrolysis, from the environmental isolates Pseudomonas putida and Agrobacterium tumefaciens. The DNA sequences analysed were capable of activating expression of the reporter enzymes, β-glucuronidase and β-galactosidase, present on pMJ445, indicating the presence of divergent promoters in the sequences selected. In addition, we demonstrated that pMJ445 can be applied to gene regulation studies.
Description
CITATION: Jiwaji, M., Matcher, G. F. & Dorrington, R. A. 2008. A broad host range reporter plasmid for the analysis of divergent promoter regions. South African Journal of Science, 104:305-307.
The original publication is available at https://www.sajs.co.za
The original publication is available at https://www.sajs.co.za
Keywords
Agrobacterium tumefaciens, Gram-negative bacteria, Pseudomonas putida, Escherichia coli
Citation
Jiwaji, M., Matcher, G. F. & Dorrington, R. A. 2008. A broad host range reporter plasmid for the analysis of divergent promoter regions. South African Journal of Science, 104:305-307.