Potential of entomopathogenic nematodes for the control of the banded fruit weevil, Phlyctinus callosus (Schonherr) (Coleoptera: Curculionidae)
Date
2013-02
Authors
Journal Title
Journal ISSN
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Publisher
Cambridge University Press - CUP
Abstract
Entomopathogenic nematodes (EPN) were evaluated for their potential use as
biological control agents against Phlyctinus callosus, the banded fruit weevil
(BFW). The susceptibility of larvae and adults to EPN was evaluated using 400
infective juveniles (IJ) per insect after 4 days in 24-well bioassay trays. The
nematode isolates used were all able to infect BFW, although the larvae were
found to be more susceptible than were the adults. The percentage mortality for
BFW larvae ranged from 41 to 73% and for BFWadults from 13 to 45%. The most
effective isolate, SF41 of Heterorhabditis zealandica, was used to investigate the
effect of vertical movement of nematodes in sand and sandy loam soil, at
specified concentration and temperature. A higher (82.2 ± 0.084%) percentage
mortality rate was obtained with the sandy loam soil, than with the use of sand
(67.5 ± 0.12%). The LD50 and LD90 values after 4 days of incubation were 96 and
278 IJ/50 µl, respectively. Nematodes were inactive below 15ºC, with the highest
mortality of 74 ± 0.081% for BFW larvae recorded at 25ºC. Heterorhabditis
zealandica was able to complete its life cycle successfully in sixth-instar BFW
larvae after a period of 22 days. The study showed BFW larvae not to be as
susceptible to nematode infection as they need a high concentration (400 IJ/larva)
and 4 days to give effective control.
Description
The original publication is available at http://journals.cambridge.org/action/displayJournal?jid=JHL
Keywords
Fruit -- Diseases and pests -- Biological control, Beetles -- Biological control, Insect nematodes, Nematodes as biological pest control agents
Citation
Ferreira, T. & Malan, A.P. 2013. Potential of entomopathogenic nematodes for the control of the banded fruit weevil, Phlyctinus callosus (Schönherr) (Coleoptera: Curculionidae). Journal of Helminthology, FirstViewArticles, doi:10.1017/S0022149X13000175.