Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts

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dc.contributor.author Habte, Habtom H.
dc.contributor.author De Beer, Corena
dc.contributor.author Lotz, Zoe E.
dc.contributor.author Roux, Paul
dc.contributor.author Mall, Anwar S.
dc.date.accessioned 2010-12-13T09:43:38Z
dc.date.available 2010-12-13T09:43:38Z
dc.date.issued 2010-10
dc.identifier.citation Habte, HH, De Beer, C, Lotz, ZE, Roux, P, & Mall, AS 2010, 'Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts', Virology Journal, 7(1):269. en_ZA
dc.identifier.issn 1743-422X
dc.identifier.other http://dx.doi.org/10.1186/1743-422X-7-269
dc.identifier.uri http://hdl.handle.net/10019.1/5060
dc.description Includes bibliography
dc.description.abstract Background: We have previously shown that MUC5B and MUC7 mucins from saliva of HIV negative individuals inhibit HIV-1 activity by 100% in an in vitro assay. The purpose of this subsequent study was to investigate whether MUC5B and MUC7 from saliva of HIV patients or with full blown AIDS had a similar inhibitory activity against the virus. Methods Salivary MUC5B and MUC7 from HIV patients with different CD4 counts (< 200, 200-400 and > 400) were incubated with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). Cells were then cultured and viral replication was measured by a qualitative p24 antigen assay. The size, charge and immunoreactivity of mucins from HIV negative and positive individuals was also analysed by SDS-PAGE, Western blot and ELISA respectively. Results: It was shown that irrespective of their CD4 counts both MUC5B and MUC7 from HIV patients, unlike the MUC5B and MUC7 from HIV negative individuals, did not inhibit HIV-1 activity. Size, charge and immunoreactivity differences between the mucins from HIV negative and positive individuals and among the mucins from HIV patients of different CD4 count was observed by SDS-PAGE, Western blot and ELISA. Conclusions: Purified salivary mucins from HIV positive patients do not inhibit the AIDS virus in an in vitro assay. Although the reason for the inability of mucins from infected individuals to inhibit the virus is not known, it is likely that there is an alteration of the glycosylation pattern, and therefore of charge of mucin, in HIV positive patients. The ability to inhibit the virus by aggregation by sugar chains is thus diminished. en_ZA
dc.format.extent 9 p. : ill.
dc.language.iso en_US en_ZA
dc.publisher BioMed Central en_ZA
dc.subject Mucin preparation, purification, identification and analysis en_ZA
dc.subject Toxicity assay en_ZA
dc.subject Inhibition assay en_ZA
dc.subject Gradient gel analyses en_ZA
dc.subject Immunoreactivity analyses en_ZA
dc.subject Western blotting analyses en_ZA
dc.subject HIV inhibition assay en_ZA
dc.subject Enzyme linked immunosorbent assay en_ZA
dc.subject.lcsh Saliva -- Microbiology en_ZA
dc.subject.lcsh Mucus -- analysis en_ZA
dc.subject.lcsh Aids (disease) -- Analysis
dc.title Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts en_ZA
dc.type Article en_ZA
dc.date.updated 2010-11-02T07:10:28Z
dc.description.version Peer Reviewed
dc.language.rfc3066 en
dc.rights.holder Habte et al.; licensee BioMed Central Ltd. en_ZA


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