Doctoral Degrees (Biochemistry)
Recent Submissions
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Quantifying the insulin response in mouse C2C12 skeletal muscle: a minimal modelling approach
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: The insulin signalling cascade is one of the most important regulatory and signalling pathways in humans. Dysregulation or dysfunction of the insulin signalling path- ways often underlies the molecular ... -
The development of standardized ion mobility and mass spectrometry metabolomics methods for the characterization of plant phenolics
(Stellenbosch : Stellenbosch University, 2021-04)ENGLISH ABSTRACT: Plants and plant-derived products contain a plethora of phenolic compounds, with a broad range of health benefits and useful applications such as in drug design. These phenolic compounds ... -
An investigation into the biosynthesis and metabolism of adrenal C11-oxy steroids
(2020-12)ENGLISH ABSTRACT: This study describes: ▪ an in vitro investigation into the biosynthesis and metabolism of 11β-hydroxyprogesterone (11OHP4) and 21-deoxycortisol (21dF) by steroidogenic enzymes which ... -
The tyrocidines in the creation of antimicrobial cellulose and sterilizing materials
(Stellenbosch : Stellenbosch University, 2020-04)ENGLISH ABSTRACT: The rising resistance of pathogenic bacteria is of great concern, especially since resistance has been reported for five of the six common hospital acquired infections. Furthermore, continual ... -
Membrane permeability and transport studies of coenzyme A, its precursors and antimetabolites.
(Stellenbosch : Stellenbosch University, 2020-04)ENGLISH ABSTRACT: Coenzyme A (CoA) is a cofactor essential to several metabolic processes, including central energy and fatty acid metabolism. In addition, there is increasing evidence that CoA also plays an important ... -
An investigation into the biosynthesis, metabolism and activity of 11-oxygenated estrogens
(Stellenbosch : Stellenbosch University, 2020-04)ENGLISH ABSTRACT: Within recent years, a growing number of studies have implicated 11-oxygenated androgens as important role players in women’s health. Significantly, 11-ketotestosterone (11KT) circulates at levels equal ... -
Computational modelling of steroid hormone biosynthesis and metabolism
(Stellenbosch : Stellenbosch University, 2020-04)ENGLISH ABSTRACT: This study describes the use of computational modelling and statistical techniques to address three topics in the field of steroid hormone research. The first is the hypocortisolism seen in the South ... -
Mathematical modelling of glycolysis in skeletal muscle cells
(Stellenbosch : Stellenbosch University, 2020-04)ENGLISH ABSTRACT: Diabetes is a term used to describe a group of metabolic diseases characterised by high blood glucose levels. Regulation of blood glucose levels is facilitated by the insulin stimulation of target tissues, ... -
Identification and genomic distribution of select combinatorial post-translational modification patterns of Trypanosoma brucei histones
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT:Trypanosoma brucei is a dixenous parasitic protozoan, and the causative agent of African Sleeping sickness, or Human African Trypanosomiasis. Trypanosomes diverged very early on from the main eukaryotic ... -
Synthesis, profiling and mode of action studies of PanSulfAms as inhibitors of coenzyme a biosynthesis and utilization
(Stellenbosch : Stellenbosch University, 2019-12)ENGLISH ABSTRACT: Though tremendous progress has been made in the development of antibacterial drugs, the evolution of antimicrobial resistance is a serious problem that is affecting the successful prevention and treatment ... -
The Staphylococcus aureus redoxome : characterization of enzymes involved in oxidative stress resistance and survival
(Stellenbosch : Stellenbosch University, 2019-12)ENGLISH ABSTRACT: The prevalence of antibiotic resistance is increasing at an alarming rate in South Africa, as well as globally. In particular, multi-drug resistant pathogens such as Staphylococcus aureus (MRSA) are a ... -
Measuring and Modelling Autophagic Flux
(Stellenbosch : Stellenbosch University, 2019-04)ENGLISH ABSTRACT: Introduction. Autophagy is an evolutionarily-conserved cellular process of self-digestion, wherein cytoplasm and organelles are sequestered and delivered to lysosomes for degradation. Autophagy plays a ... -
Strategies for the detection of endocrine disrupting compounds utilising recombinant oestrogen receptor ligand-binding domains and high specificity monoclonal antibodies
(Stellenbosch : Stellenbosch University, 2018-12)ENGLISH ABSTRACT: During the last two and a half decades, a large body of research has been amassed which indicate that various compounds capable of inducing hormone-like responses in humans and animals are present ... -
Investigating the mechanism of action of hormones used in hormone replacement therapy via estrogen receptor subtypes and the influence of the progesterone receptor
(Stellenbosch : Stellenbosch University, 2018-03)ENGLISH ABSTRACT: Estrogens and progestins used in conventional menopausal hormone therapy (HT) are associated with increased breast cancer risk. A diverse range of estrogens and progestins are available that mediate ... -
Homologous down-regulation of the glucocorticoid receptor is influenced by the dimerization state of the receptor
(Stellenbosch : Stellenbosch University, 2018-03)ENGLISH ABSTRACT: Glucocorticoids (GCs) remain the mainstay therapeutic choice for the treatment of inflammation, and exert their potent anti-inflammatory effects via the glucocorticoid receptor (GRα). However, the chronic ... -
The metabolism of 11β-hydroxyandrostenedione by steroidogenic enzymes yields metabolites contributing to the androgen pool in prostate cancer
(Stellenbosch : Stellenbosch University, 2018-03)ENGLISH ABSTRACT: This study describes: • The development and validation of three ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) analytical methods which were applied in the detection ... -
The influence of sutherlandia frutescens on adrenal steroid hormones and downstream receptor interactions
(Stellenbosch : Stellenbosch University, 2017-12)ENGLISH ABSTRACT: The study was aimed at: 1. linking anti-stress properties of Sutherlandia frutescens to its influence on adrenal steroid biosynthesis in terms of extracts, sutherlandioside (SU) compounds and ... -
Engineering and application of glycosidase derived biocatalysts in the study of mycothiol pathway enzymes
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: Glycosides are complex carbohydrates that are involved in essential cellular and molecular biological processes within all living organisms. In addition, certain glycosides have anti-cancer, antioxidant, ... -
The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A).
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: The study describes: . The synthesis of a racemic mixture of Compound A (2-(4-acetoxyphenyl)-2-chloro-N-methylethylammonium chloride), a synthetic analogue of the active compound isolated from the African ... -
Phylogenetics of the genus Erica and anthocyanin synthesis gene expression in Erica plukenetii
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: The drivers of species radiations are central to questions about the evolution of diversity. The flora of the Cape Floristic Region (CFR) is particularly diverse, has exceptionally high level of endemism ...
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