Doctoral Degrees (Biochemistry)
Recent Submissions
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The influence of sutherlandia frutescens on adrenal steroid hormones and downstream receptor interactions
(Stellenbosch : Stellenbosch University, 2017-12)ENGLISH ABSTRACT: The study was aimed at: 1. linking anti-stress properties of Sutherlandia frutescens to its influence on adrenal steroid biosynthesis in terms of extracts, sutherlandioside (SU) compounds and ...
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The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A).
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: The study describes: . The synthesis of a racemic mixture of Compound A (2-(4-acetoxyphenyl)-2-chloro-N-methylethylammonium chloride), a synthetic analogue of the active compound isolated from the African ...
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Phylogenetics of the genus Erica and anthocyanin synthesis gene expression in Erica plukenetii
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: The drivers of species radiations are central to questions about the evolution of diversity. The flora of the Cape Floristic Region (CFR) is particularly diverse, has exceptionally high level of endemism ...
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Partial characterization of lentil seed lipoxygenases and their impact in wheat (Triticum aestivum L.) bread making
(Stellenbosch : Stellenbosch University, 2017-03)ENGLISH ABSTRACT: This study describes: 1. The evaluation of lentil seed flour as an alternative for soybean seed flour; as a source of lipoxygenase during the making of white bread. 2. The development of efficient, and ...
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Development of an integrated metabolic analysis toolbox
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: Life is arguably the most complex of all natural phenomena, yet it arises from essentially dead molecular components. The goal of systems biology is to be able to understand how the properties and ...
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Biofilms as multifunctional surface coatings and adaptive systems: a biomimetic approach
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: Biomimicry is an emerging scientific discipline that promotes nature-inspired innovation for sustainable solutions. Several patterns and survival strategies are repeated in Nature and these have been ...
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A high rate biofilm contact reactor for winery wastewater treatment
(Stellenbosch : Stellenbosch University, 2016-12)ENGLISH ABSTRACT: Winemaking produces variable volumes wastewater rich in biodegradable organic material, with fluctuating chemical composition and pH values according to the seasonal activities of the cellar. Releasing ...
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Optimised bacterial production and characterisation of natural antimicrobial peptides with potential application in agriculture
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH ABSTRACT : The loss incurred due to microbial spoilage is a great concern to the agricultural and food industries. Microbial pathogens have typically been controlled through the use of chemical control agents and ...
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A comparative analysis of CoA biosynthesis in selected organisms: a metabolite study
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH SUMMARY: This study investigated the biochemical regulation of CoA production because it is an essential pathway that presents an important target for antimicrobial drug discovery studies. Currently, the specific ...
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The development of a DNA vaccine against Mycoplasma nasistruthionis sp. nov. for use in ostriches
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: Mycoplasma nasistruthionis sp. nov. str. Ms03 (Ms03) is one of three Mycoplasma species that were identified from ostriches. Mycoplasmas infections have been implicated in ostrich chick mortalities, ...
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A biochemical study of the effect of ultraviolet treatment on bovine milk and Cheddar cheese
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: This study describes: 1. The evaluation of a novel, patented thin-film, turbulent-flow Ultravioletdisinfection system as an alternative processing method to thermal pasteurisationfor the disinfection of ...
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Coenzyme A biosynthesis and Coenzyme A-dependent redox processes as targets for anti-staphylococcal drug development
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH SUMMARY: Staphylococcus aureus, the bacterium that causes most hospital-acquired in humans is rapidly becoming more prevalent in the community and, alarmingly, increasingly resistant to the current arsenal of ...
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The inhibition of adrenal steroidogenic enzymes and modulation of glucocorticoid levels in vitro and in vivo by aspalathus linearis (rooibos)
(Stellenbosch : Stellenbosch University, 2015-04)ENGLISH ABSTRACT: This study describes: • the influence of a methanolic extract of unfermented Rooibos and five major Rooibos flavonoids, aspalathin, nothofagin, rutin, orientin and vitexin, on the activities of key ...
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Coenzyme a biosynthesis and utilization in Plasmodium falciparum : drug targets for antimalarial chemotherapy
(Stellenbosch : Stellenbosch University, 2014-12)ENGLISH ABSTRACT: Pantothenate (also known as Vitamin B5) is the sole precursor of the essential enzyme cofactor coenzyme A (CoA) that is required in several metabolic reactions virtually in all living organisms including ...
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Phytoestrogenic extracts of Cyclopia modulate molecular targets involved in the prevention and treatment of breast cancer
(Stellenbosch : Stellenbosch University, 2014-04)ENGLISH ABSTRACT: Phytoestrogen containing extracts of Cyclopia, an indigenous South African fynbos plant used to prepare honeybush tea, may serve as a source of new estrogen analogues. It would be of great benefit if these ...
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Tyrocidines, cyclic decapeptides produced by soil bacilli, as potent inhibitors of fungal pathogens
(Stellenbosch : Stellenbosch University, 2014-04)ENGLISH ABSTRACT: The global rise in microbial resistance, ranging from the agricultural industry to the medical sector, has created the urgent need for novel or supplementary antibiotics. Antimicrobial peptides or ...
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Characterisation of small cyclic peptides with antilisterial and antimalarial activity
(Stellenbosch : Stellenbosch University, 2014-04)ENGLISH ABSTRACT: Antimicrobial peptides (AMPs) are currently the most researched group of compounds for new antimicrobial drugs especially with the rise in resistance to almost all available drugs by public health ...
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The synthesis and characterisation of analogues of the antimicrobial peptide iturin A₂
(Stellenbosch : Stellenbosch University, 1998-12)ENGLISH ABSTRACT: Iturin A, an antifungal lipopeptide, is produced by Bacillus subtilis. This cyclic peptide consists of seven D- and L-amino acid residues (L-Asn2-D-Tyr3-D-Asn4-L-Gln5-L-Pro6-DAsn7- L-Ser8) and a beta-amino ...
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Understanding glycolysis in Escherichia coli : a systems approach using nuclear magnetic resonance spectroscopy
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: This dissertation explores the behaviour and regulation of central carbon metabolism in Escherichia coli K12 W3110 under fermentative microaerobic conditions. To achieve this, an integrative systems modelling ...
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Chemopreventive properties of South African herbal teas, rooibos (Aspalathus linearis) and honeybush (Cyclopia spp) : mechanisms against skin carcinogenesis
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: The present study employed a two-phased approach to investigate the possible mechanisms involved in the chemopreventive properties of rooibos (Aspalathus linearis) and different honeybush species (Cyclopia ...
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Altered lipid metabolism as a possible mechanism in fumonisin-induced hepatocarcinogenesis in rats and investigations into risk assessment in humans
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: Exposure to food contaminates such as mycotoxins have been associated with a variety of animal and human diseases worldwide. In South Africa, maize is the most To further refine risk assessment in the ...
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A kinetic model of glucose catabolism in Plasmodium falciparum
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Malaria infects over 200 million individuals and leads to the death of over 600 000 people annually. Currently artemisinin combination therapy treatments are effective in treating the disease, but ...
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The regulatory design of glycogen metabolism in mammalian skeletal muscle
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: It is widely accepted that insufficient insulin-stimulated activation of muscle glycogen synthesis is one of the major components of non-insulin-dependent (type 2) diabetes mellitus. Glycogen synthase, ...
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A study of the molecular mechanism of progestin-induced regulation of IL-12 and IL-10 and implications for HIV pathogenesis
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Medroxyprogesterone acetate (MPA) and norethisterone (NET) and its derivatives (norethisterone enanthate (NET-EN); norethisterone acetate (NET-A)), designed to mimic the actions of the endogenous hormone ...
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A study of the strain evolution and recombination of South African isolates of Potato virus Y
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: Potato virus Y (PVY) is responsible for considerable yield losses in the South African potato industry. The incidence of this virus has greatly increased over the past 20 years. In previous studies ...
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The influence of 3βHSD on adrenal steroidogenesis and the factors which influence its activity
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: This study describes: - the characterization and comparison of the enzymatic activity of both Angora and ovine 3βHSD expressed in non-steroidogenic COS-1 cells. The apparent Km and Vmax values for ...
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An investigation into the molecular mechanism of action of the progestins, medroxyprogesterone acetate and norethisterone acetate
(Stellenbosch : Stellenbosch University, 2004-04)ENGLISH ABSTRACT: Although the progestins medroxyprogesterone acetate (MPA) and norethisterone acetate (NET-A) are widely used in reproductive therapy, the steroid receptors and their target genes involved in the actions ...
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Immunological and epidemiological investigations in South African ostriches and penguins
(Stellenbosch : Stellenbosch University, 2004-04)ENGLISH ABSTRACT: Newcastle disease (NO) and mycoplasma infections in ostriches have considerable economic implications for the South African ostrich industry in that NO is a limiting factor in the export of ostrich ...
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Investigation of glucocorticoid and dissociated glucocorticoid activity in hepatoma cell lines with specific reference to regulation of the corticosteroid binding globulin (CBG) proximal promoter'
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: This study investigated the effect of several hormones on the rat corticosteroid binding globulin proximal promotor and for the first time showed that modulation occurs at the promotor level and can ...
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Differential protein expression focusing on the mannose phosphotransferase system, in Listeria monocytogenes strains with class 11a bacteriocin resistance
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: Please refer to fulltext for abstract
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