Doctoral Degrees (Biochemistry)
Recent Submissions
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Optimised bacterial production and characterisation of natural antimicrobial peptides with potential application in agriculture
(Stellenbosch : Stellenbosch University, 2016-03)ENGLISH ABSTRACT : The loss incurred due to microbial spoilage is a great concern to the agricultural and food industries. Microbial pathogens have typically been controlled through the use of chemical control agents and ...
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The development of a DNA vaccine against Mycoplasma nasistruthionis sp. nov. for use in ostriches
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: Mycoplasma nasistruthionis sp. nov. str. Ms03 (Ms03) is one of three Mycoplasma species that were identified from ostriches. Mycoplasmas infections have been implicated in ostrich chick mortalities, ...
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A biochemical study of the effect of ultraviolet treatment on bovine milk and Cheddar cheese
(Stellenbosch : Stellenbosch University, 2015-12)ENGLISH ABSTRACT: This study describes: 1. The evaluation of a novel, patented thin-film, turbulent-flow Ultravioletdisinfection system as an alternative processing method to thermal pasteurisationfor the disinfection of ...
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The inhibition of adrenal steroidogenic enzymes and modulation of glucocorticoid levels in vitro and in vivo by aspalathus linearis (rooibos)
(Stellenbosch : Stellenbosch University, 2015-04)ENGLISH ABSTRACT: This study describes: • the influence of a methanolic extract of unfermented Rooibos and five major Rooibos flavonoids, aspalathin, nothofagin, rutin, orientin and vitexin, on the activities of key ...
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Phytoestrogenic extracts of Cyclopia modulate molecular targets involved in the prevention and treatment of breast cancer
(Stellenbosch : Stellenbosch University, 2014-04)ENGLISH ABSTRACT: Phytoestrogen containing extracts of Cyclopia, an indigenous South African fynbos plant used to prepare honeybush tea, may serve as a source of new estrogen analogues. It would be of great benefit if these ...
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Tyrocidines, cyclic decapeptides produced by soil bacilli, as potent inhibitors of fungal pathogens
(Stellenbosch : Stellenbosch University, 2014-04)ENGLISH ABSTRACT: The global rise in microbial resistance, ranging from the agricultural industry to the medical sector, has created the urgent need for novel or supplementary antibiotics. Antimicrobial peptides or ...
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Characterisation of small cyclic peptides with antilisterial and antimalarial activity
(Stellenbosch : Stellenbosch University, 2014-04)ENGLISH ABSTRACT: Antimicrobial peptides (AMPs) are currently the most researched group of compounds for new antimicrobial drugs especially with the rise in resistance to almost all available drugs by public health ...
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The synthesis and characterisation of analogues of the antimicrobial peptide iturin A₂
(Stellenbosch : Stellenbosch University, 1998-12)ENGLISH ABSTRACT: Iturin A, an antifungal lipopeptide, is produced by Bacillus subtilis. This cyclic peptide consists of seven D- and L-amino acid residues (L-Asn2-D-Tyr3-D-Asn4-L-Gln5-L-Pro6-DAsn7- L-Ser8) and a beta-amino ...
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Understanding glycolysis in Escherichia coli : a systems approach using nuclear magnetic resonance spectroscopy
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: This dissertation explores the behaviour and regulation of central carbon metabolism in Escherichia coli K12 W3110 under fermentative microaerobic conditions. To achieve this, an integrative systems modelling ...
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Chemopreventive properties of South African herbal teas, rooibos (Aspalathus linearis) and honeybush (Cyclopia spp) : mechanisms against skin carcinogenesis
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: The present study employed a two-phased approach to investigate the possible mechanisms involved in the chemopreventive properties of rooibos (Aspalathus linearis) and different honeybush species (Cyclopia ...
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Altered lipid metabolism as a possible mechanism in fumonisin-induced hepatocarcinogenesis in rats and investigations into risk assessment in humans
(Stellenbosch : Stellenbosch University, 2013-12)ENGLISH ABSTRACT: Exposure to food contaminates such as mycotoxins have been associated with a variety of animal and human diseases worldwide. In South Africa, maize is the most To further refine risk assessment in the ...
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A kinetic model of glucose catabolism in Plasmodium falciparum
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Malaria infects over 200 million individuals and leads to the death of over 600 000 people annually. Currently artemisinin combination therapy treatments are effective in treating the disease, but ...
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The regulatory design of glycogen metabolism in mammalian skeletal muscle
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: It is widely accepted that insufficient insulin-stimulated activation of muscle glycogen synthesis is one of the major components of non-insulin-dependent (type 2) diabetes mellitus. Glycogen synthase, ...
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A study of the molecular mechanism of progestin-induced regulation of IL-12 and IL-10 and implications for HIV pathogenesis
(Stellenbosch : Stellenbosch University, 2013-03)ENGLISH ABSTRACT: Medroxyprogesterone acetate (MPA) and norethisterone (NET) and its derivatives (norethisterone enanthate (NET-EN); norethisterone acetate (NET-A)), designed to mimic the actions of the endogenous hormone ...
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A study of the strain evolution and recombination of South African isolates of Potato virus Y
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: Potato virus Y (PVY) is responsible for considerable yield losses in the South African potato industry. The incidence of this virus has greatly increased over the past 20 years. In previous studies ...
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The influence of 3βHSD on adrenal steroidogenesis and the factors which influence its activity
(Stellenbosch : Stellenbosch University, 2012-12)ENGLISH ABSTRACT: This study describes: - the characterization and comparison of the enzymatic activity of both Angora and ovine 3βHSD expressed in non-steroidogenic COS-1 cells. The apparent Km and Vmax values for ...
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An investigation into the molecular mechanism of action of the progestins, medroxyprogesterone acetate and norethisterone acetate
(Stellenbosch : Stellenbosch University, 2004-04)ENGLISH ABSTRACT: Although the progestins medroxyprogesterone acetate (MPA) and norethisterone acetate (NET-A) are widely used in reproductive therapy, the steroid receptors and their target genes involved in the actions ...
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Immunological and epidemiological investigations in South African ostriches and penguins
(Stellenbosch : Stellenbosch University, 2004-04)ENGLISH ABSTRACT: Newcastle disease (NO) and mycoplasma infections in ostriches have considerable economic implications for the South African ostrich industry in that NO is a limiting factor in the export of ostrich ...
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Investigation of glucocorticoid and dissociated glucocorticoid activity in hepatoma cell lines with specific reference to regulation of the corticosteroid binding globulin (CBG) proximal promoter'
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: This study investigated the effect of several hormones on the rat corticosteroid binding globulin proximal promotor and for the first time showed that modulation occurs at the promotor level and can ...
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Differential protein expression focusing on the mannose phosphotransferase system, in Listeria monocytogenes strains with class 11a bacteriocin resistance
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: Please refer to fulltext for abstract
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Changes in cell surface and metabolism associated with strains of Listeria monocytogenes displaying different sensitivities to class IIa bacteriocins
(Stellenbosch : Stellenbosch University, 2003-12)ENGLISH ABSTRACT: The possible use of the bacterially produced antimicrobial peptides, and in particular class IIa bacteriocins as food preservatives is a motivating factor in studies on resistance to them by food-borne ...
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Isolation and characterisation of a polygalacturonase-inhibiting protein (PGIP) and its encoding gene from Vitis vinifera L.
(Stellenbosch : Stellenbosch University, 2001-12)ENGLISH ABSTRACT: Polygalacturonase-inhibiting proteins (PGIPs) are present in the cell walls of a variety of plant species. These proteins have been shown to specifically inhibit endopolygalacturonases (endo-PGs) ...
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Cloning and identification of genes involved in the interaction between the bacterial stone fruit pathogen Pseudomonas syringae pv. syringae strain NV and plum trees
(Stellenbosch : Stellenbosch University, 2001-03)ENGLISH ABSTRACT: Bacterial canker of stone fruit, caused by Pseudomonas syringae pv. syringae, is one of the most destructive crop diseases in South Africa. Chemical control has failed completely and effective long-term ...
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The role of nitrogen in the regulation of microcystin content in Microcystis aeruginosa
(Stellenbosch : Stellenbosch University, 2005-12)ENGLISH ABSTRACT: Several genera of cyanobacteria produce a range of toxins. The increased rate of eutrophication of surface fresh waters due to anthropogenic inputs has resulted in more frequent and severe cyanobacterial ...
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Simulation and database software for computational systems biology : PySCes and JWS Online
(Stellenbosch : Stellenbosch University, 2005-03)ENGLISH ABSTRACT: Since their inception, biology and biochemistry have been spectacularly successful in characterising the living cell and its components. As the volume of information about cellular components continues ...
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Investigation of malt factors that influence beer production and quality
(Stellenbosch : Stellenbosch University, 2005-03)ENGLISH ABSTRACT: A number of relevant brewing industry issues associated with malt quality were examined. These included beer foam quality, premature flocculation of yeast during fermentation and antimicrobial factors ...
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Investigation of complexation and antimicrobial activity of gramicidin S in the presence of lipopeptides from Bacillus subtilis
(Stellenbosch : Stellenbosch University, 2005-03)ENGLISH ABSTRACT: The implication of biologically active peptides from different organisms on one another in complex ecological communities is largely unknown at this stage. The elucidation of the nature of this influence ...
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An investigation of heterologous expression of human steroidogenic cytochromes P450 in yeasts
(Stellenbosch : Stellenbosch University, 2005-04)ENGLISH ABSTRACT: This study: 1. Compares various heterologous expression systems for high-level expression of cytochromes P450. Limitations of the existing cytochromes P450 expression systems are discussed and possibilities ...
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Cancer modulating properties of unique South African herbal teas (rooibos and honeybush) in short term in vitro and in vivo carcinogenesis assays
(Stellenbosch : Stellenbosch University, 2004-12)ENGLISH ABSTRACT: This thesis provides the first scientific evidence on the cancer modulating properties of two unique South African herbal teas, rooibos (Aspalathus Iinearis) and honeybush (Cyclopia intermedia) utilizing ...
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Identification of candidate genes and testing for association with tuberculosis in humans
(Stellenbosch : Stellenbosch University, 2007-12)ENGLISH ABSTRACT: This research investigated human candidate genes for susceptibility to tuberculosis and the effect of various factors on time to sputum conversion in the admixed South African Coloured (SAC) population. ...
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