Maternal and fetal single nucleotide polymorphisms in the epoxide hydrolase and gluthatione S-transferase P1 genes are not associated with pre-eclampsia in the Coloured population of the Western Cape, South Africa
Date
2004
Authors
Gerhardt G.S.
Peters W.H.M.
Hillermann R.
Odendaal H.J.
Carelse-Tofa K.
Raijmakers M.T.M.
Steegers E.A.P.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Oxidative stress is thought to play an important role in the pathophysiology of pre-eclampsia. A defect in certain enzymes responsible for detoxification may cause prolonged exposure to reactive by-products and contribute to maternal endothelial as well as placental damage. Two polymorphisms affecting the function of the biotransformation enzymes epoxide hydrolase and glutathione S-transferase P1 were shown previously to be associated with pre-eclampsia in a Dutch population. The aim of this study was to determine if these two polymorphisms (maternal or fetal) contribute to pre-eclampsia in an anthropologically distinct population (the Western Cape region of South Africa) with a high incidence of the disease. Genomic DNA of mother-infant pairs with severe pre-eclampsia (n = 144), a population control group (n = 156) and control mother-infant pairs with uncomplicated pregnancy outcome (n = 45) were analysed for the EPHX and GSTP1 polymorphisms by polymerase chain reaction amplification and restriction enzyme digestion. Each polymorphism had a similar distribution in case and control subjects (mother and infant). The Val105/Val105 genotype of GSTP1 occurred at a higher frequency than reported for other populations. Neither maternal nor fetal EPHX Tyr113His and GSTP1 Ile105Val polymorphisms appear to contribute significantly to the pathophysiology of pre-eclampsia in the Coloured population of the Western Cape region of South Africa. © Taylor & Francis Limited, 2004.
Description
Keywords
DNA, epoxide hydrolase, glutathione transferase, adolescent, adult, anthropology, article, digestion, disease association, disease severity, female, gene amplification, genetic variability, genomics, human, major clinical study, mother child relation, oxidative stress, pathophysiology, polymerase chain reaction, preeclampsia, pregnancy, priority journal, single nucleotide polymorphism, South Africa, African Continental Ancestry Group, Alleles, DNA, Epoxide Hydrolases, Exons, Female, Fetus, Gene Frequency, Genotype, Glutathione Transferase, Histidine, Humans, Polymorphism, Single Nucleotide, Pre-Eclampsia, Pregnancy, South Africa
Citation
Journal of Obstetrics and Gynaecology
24
8
24
8