Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF
Date
2001
Authors
Esterhuizen A.D.
Franken D.R.
Lourens J.G.H.
Van Rooyen L.H.
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Abstract
The study aimed to establish zona pellucida induced acrosome reaction response (ZIAR) among 35 couples with normal and G-pattern sperm morphology and repeated poor fertilization results during assisted reproduction treatment. ZIAR tests were performed using 0.25 zona pellucida/μl co-incubated with spermatozoa for 60 min. Acrosome reactions were measured with FITC-PSA staining, and expressed as the difference between stimulated and unstimulated (spontaneous) sperm populations. Results were compared with IVF rates of metaphase II oocytes. Interactive dot diagrams divided the patients into two groups, i.e. ZIAR <15% and ZIAR > 15%, with mean fertilization rates of 49 and 79% respectively. The sensitivity and specificity for ZIAR results versus fertilization were 93 and 100% respectively. The area under the curve was 99% and the 95% confidence interval did not include 0.5 which implies that the ZIAR test is able to predict fertilization failure among IVF patients. In conclusion, the ZIAR test has diagnostic potential since it can assist the clinician to identify couples that will benefit from intracytoplasmic sperm injection therapy.
Description
Keywords
acrosome reaction, article, clinical feature, controlled study, female, fertilization, fertilization in vitro, function test, human, human tissue, infertility, major clinical study, male, prediction, semen analysis, technique, treatment failure, zona pellucida, Acrosome Reaction, Adult, Female, Fertilization in Vitro, Humans, Infertility, Female, Male, Predictive Value of Tests, ROC Curve, Sperm Count, Sperm Injections, Intracytoplasmic, Sperm Motility, Treatment Failure, Zona Pellucida
Citation
Human Reproduction
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