Deletion mapping of 39 random isolated Y-chromosome DNA fragments
Date
1990
Authors
Oosthuizen C.J.J.
Herbert J.S.
Vermaak L.K.
Brusnicky J.
Fricke J.
Du Plessis L.
Retief A.E.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Thirty-nine recombinants isolated from a Y chromosome-specific library were deletion mapped. Seven deletion intervals were defined by hybridization of probes to DNA of eight individuals with aberrant Y chromosomes. Extreme cytogenetic limits of the deletion intervals were determined by in situ hybridization of one probe per deletion interval. Five intervals, with a total of twenty-five probes, were allocated to the long-arm euchromatic region. The probes described will be useful for characterization of aberrant Y chromosomes, in searching for expressed sequences on the Y chromosome, and for further study of the evolutionary relationship between the Y chromosome and other chromosomes.
Description
Keywords
dna fragment, article, dna library, dna probe, gene deletion, gene mapping, genetic engineering, heredity, human, human cell, normal human, normal value, priority journal, Y chromosome, Chromosome Deletion, Chromosome Mapping, DNA Probes, Human, Male, Nucleic Acid Hybridization, Ring Chromosomes, Support, U.S. Gov't, Non-P.H.S., Y Chromosome
Citation
Human Genetics
85
2
85
2