The zona pellucida-induced acrosome reaction of human spermatozoa involves extracellular signal-regulated kinase activation
Date
2001, 2001
Authors
Du Plessis S.S.
Page C.
Franken D.R.
Du Plessis S.S.
Page C.
Franken D.R.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Extracellular signal-regulated kinases(ERKs), belonging to the family of mitogen-activated protein kinases (MAPKs), are cytoplasmic and nuclear serine/threonine kinases involved in the signal transduction of several extracellular effectors. Recent evidence indicates the presence of p21 Ras and the phosphorylation of ERK1 and ERK2, suggesting the occurrence of the Ras/ERK cascade in mammalian spermatozoa. The present article describes the biological role of ERK during the acrosome reaction of human spermatozoa on stimulation with zona pellucida (ZP). The mitogen-activated protein-kinase inhibitor PD098059 was used as a pharmacological tool to study the involvement of extracellular signal-regulated kinases in the induction of the acrosome reaction in human spermatozoa. This compound significantly inhibited the acrosome reaction induced by both ZP and the calcium ionophore A23187. These results suggest that ERKs are involved in the signal trans-duction pathway through which ZP stimulation works during the process of fertilization.
Extracellular signal-regulated kinases(ERKs), belonging to the family of mitogen-activated protein kinases (MAPKs), are cytoplasmic and nuclear serine/threonine kinases involved in the signal transduction of several extracellular effectors. Recent evidence indicates the presence of p21 Ras and the phosphorylation of ERK1 and ERK2, suggesting the occurrence of the Ras/ERK cascade in mammalian spermatozoa. The present article describes the biological role of ERK during the acrosome reaction of human spermatozoa on stimulation with zona pellucida (ZP). The mitogen-activated protein-kinase inhibitor PD098059 was used as a pharmacological tool to study the involvement of extracellular signal-regulated kinases in the induction of the acrosome reaction in human spermatozoa. This compound significantly inhibited the acrosome reaction induced by both ZP and the calcium ionophore A23187. These results suggest that ERKs are involved in the signal trans-duction pathway through which ZP stimulation works during the process of fertilization.
Extracellular signal-regulated kinases(ERKs), belonging to the family of mitogen-activated protein kinases (MAPKs), are cytoplasmic and nuclear serine/threonine kinases involved in the signal transduction of several extracellular effectors. Recent evidence indicates the presence of p21 Ras and the phosphorylation of ERK1 and ERK2, suggesting the occurrence of the Ras/ERK cascade in mammalian spermatozoa. The present article describes the biological role of ERK during the acrosome reaction of human spermatozoa on stimulation with zona pellucida (ZP). The mitogen-activated protein-kinase inhibitor PD098059 was used as a pharmacological tool to study the involvement of extracellular signal-regulated kinases in the induction of the acrosome reaction in human spermatozoa. This compound significantly inhibited the acrosome reaction induced by both ZP and the calcium ionophore A23187. These results suggest that ERKs are involved in the signal trans-duction pathway through which ZP stimulation works during the process of fertilization.
Description
Keywords
calcimycin; calcium ionophore; mitogen activated protein kinase; mitogen activated protein kinase inhibitor; serine; threonine; acrosome reaction; article; controlled study; enzyme activation; female; fertilization; human; human cell; male; normal human; phosphorylation; signal transduction; spermatozoon; zona pellucida; Acrosome; Ca(2+)-Calmodulin Dependent Protein Kinase; Calcimycin; Enzyme Activation; Enzyme Inhibitors; Female; Flavonoids; Humans; Ionophores; Male; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; Sperm Capacitation; Sperm-Ovum Interactions; Spermatozoa; Zona Pellucida, calcimycin, calcium ionophore, mitogen activated protein kinase, mitogen activated protein kinase inhibitor, serine, threonine, acrosome reaction, article, controlled study, enzyme activation, female, fertilization, human, human cell, male, normal human, phosphorylation, signal transduction, spermatozoon, zona pellucida, Acrosome, Ca(2+)-Calmodulin Dependent Protein Kinase, Calcimycin, Enzyme Activation, Enzyme Inhibitors, Female, Flavonoids, Humans, Ionophores, Male, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases, Sperm Capacitation, Sperm-Ovum Interactions, Spermatozoa, Zona Pellucida
Citation
Andrologia
33
6
Andrologia
33
6
33
6
Andrologia
33
6