Molecular analysis of the malic enzyme gene (mae2) of Schizosaccharomyces pombe

Date
1994
Authors
Viljoen M.
Subden R.E.
Krizus A.
Van Vuuren H.J.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Sequence analysis of a 4.6-kb HindIII fragment containing the malic enzyme gene (mae2) of Schizosaccharomyces pombe, revealed the presence of an open reading frame of 1695 nucleotides, coding for a 565 amino acid polypeptide. The mae2 gene is expressed constitutively and encodes a single mRNA transcript of 2.0 kb. The mae2 gene was mapped on chromosome III by chromoblotting. The coding region and inferred amino acid sequence showed significant homology with 12 malic enzyme genes and proteins from widely different origins. Eight highly homologous regions were found in these malic enzymes, suggesting that they contain functionally conserved amino acid sequences that are indispensable for activity of malic enzymes. Two of these regions have previously been reported to be NAD- and NADP-binding sites.
Description
Keywords
malate dehydrogenase (decarboxylating), article, chromosome 3, dna sequence, gene mapping, gene structure, nonhuman, priority journal, schizosaccharomyces pombe, sequence homology, wine, Amino Acid Sequence, Base Sequence, Blotting, Northern, Chromosome Mapping, DNA Probes, Genes, Fungal, Malate Dehydrogenase, Molecular Sequence Data, NAD, NADP, Schizosaccharomyces, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Support, Non-U.S. Gov't
Citation
Yeast
10
5