The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids
Date
1995
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Abstract
The mae1 gene of the yeast Schizosaccharomyces pombe was identified on the basis of its ability to complement a mutant defective in the transport of malic acid. Analysis of the DNA sequence revealed an open reading frame of 1314 base pairs, encoding a polypeptide of 438 amino acids with a predicted molecular weight of 49 kDa. A hydropathy profile of the predicted amino acid sequence revealed a protein with ten membrane-spanning or associated domains and hydrophilic N- and C- termini. The predicted secondary structure of the protein is similar to models proposed for other integral membrane proteins from both prokaryotes and eukaryotes. The S. pombe mae1 gene encodes a single mRNA of 1.5 kb. The mae1 gene is expressed constitutively and is not subject to catabolite repression as was previously reported for the malate permease systems of Candida utilis and Hansenula anomala. The mae1 gene was mapped 2842 bp 5' to the MFm1 gene on chromosome I. Transport assays revealed that the mae1 gene encodes a permease involved in the uptake of L-malate, succinate and malonic acid. The sequence of the S. pombe mae1 gene is available in GenBank under Accession Number U21002.
Description
Keywords
dicarboxylic acid, malic acid, malonic acid, permease, succinic acid, article, cell membrane permeability, dna sequence, drug transport, enzyme structure, gene expression, gene mapping, genetic analysis, priority journal, schizosaccharomyces pombe, Amino Acid Sequence, Base Sequence, Biological Transport, Active, Chromosomes, Fungal, Cloning, Molecular, Dicarboxylic Acids, DNA, Fungal, Fermentation, Genes, Fungal, Malates, Membrane Transport Proteins, Molecular Sequence Data, Open Reading Frames, Protein Structure, Secondary, Restriction Mapping, Schizosaccharomyces, Support, Non-U.S. Gov't, Candida, Eukaryota, Pichia anomala, Pichia jadinii, Prokaryota, Schizosaccharomyces pombe
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Yeast
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