Differentiation of Leuconostoc species by nicotinamide adenine dinucleotide-dependent D(-)-lactic dehydrogenase profiles
Date
1990
Authors
Dicks L.M.T.
Van Vuuren H.J.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The electrophoretic mobility of the D(-)-nLDH's of Leuconostoc oenos (nine strains), Leuconostoc mesenteroides subsp. mesenteroides (one strain), Leuconostoc mesenteroides subsp. dextranicum (one strain), Leuconostoc lactis (three strains), Leuconostoc paramesenteroides (two strains) and a Leuconostoc sp. were compared using conventional and stacked polyacrylamide gels. A single diffused D(-)-nLDH band was obtained for each strain on a 7% acrylamide gel. However, several well defined D(-)-nLDH bands were obtained for each strain when a polyacrylamide stacked gel was used. Based on D(-)-nLDH profiles, strains of L. oenos were divided into two subgroups. The overall D(-)-nLDH profiles of strains of L. oenos were different from those obtained for the non-acidophilic Leuconostoc spp. Leuconostoc mesenteroides subsp. mesenteroides and L. mesenteroides subsp. dextranicum had similar D(-)-nLDH profiles. The overall D(-)-nLDH profiles of L. lactis corresponded well. Different D(-)-nLDH profiles were obtained for L. paramesenteroides DSM 20201 and the type strain of L. paramesenteroides (DSM 20288(T)). The D(-)-nLDH profile of Leuconostoc sp. ATCC 21436 corresponded well with the profile obtained for the D(-)-nLDH electrophoretic marker of Lactobacillus leichmannii.
Description
Keywords
lactate dehydrogenase, nicotinamide adenine dinucleotide, article, bacterium identification, leuconostoc, nonhuman, priority journal, Bacteria (microorganisms), Lactobacillus leichmannii, Leuconostoc, Leuconostoc lactis, Leuconostoc mesenteroides subsp. dextranicum, Leuconostoc mesenteroides subsp. mesenteroides, Oenococcus oeni, Weissella paramesenteroides
Citation
FEMS Microbiology Letters
67
02-Jan
67
02-Jan