Mutation of Gly-444 inactivates the S. pombe malic enzyme

Viljoen M. ; Van Der Merwe M. ; Subden R.E. ; Van Vuuren H.J.J. (1998)

Article

A mutant malic enzyme gene, mae2-, was cloned from a strain of Schizosaccharomyces pombe that displayed almost no malic enzyme activity. Sequence analysis revealed only one codon-altering mutation, a guanine to adenine at nucleotide 1331, changing the glycine residue at position 444 to an aspartate residue. Gly-444 is located in Region H, previously identified as one of eight highly conserved regions in malic enzymes. We found that Gly-444 is absolutely conserved in 27 malic enzymes from various prokaryotic and eukaryotic sources, as well as in three bacterial malolactic enzymes investigated. The evolutionary conservation of Gly-444 suggests that this residue is important for enzymatic function. Copyright (C) 1998 Federation of European Microbiological Societies.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/12904
This item appears in the following collections: