Molecular and phylogenetic analysis of HIV type 1 vpr sequences of South African strains
Date
2009
Authors
Romani B.
Glashoff R.
Engelbrecht S.
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Abstract
HIV-1 subtype C is the prevalent subtype in South Africa, with non-C subtypes being sporadically detected. The accessory genes of subtype C have not been well studied in South Africa. In this study the HIV-1 vpr region of 58 strains was amplified, sequenced, and subtyped. Phylogenetic analysis characterized 54 strains as HIV-1 subtype C and 4 strains as HIV-1 subtype B. The amino acid sequence of the protein was also investigated. The overall amino acid sequence of Vpr was conserved as well as the motifs FPRPWL (34-39: IH1) and TYGDTW (49-54: IH2). The C-terminal was more variable. The importance of these motifs and variability needs to be addressed. © Mary Ann Liebhert, Inc.
Description
Keywords
virus RNA, Vpr protein, vpr protein, Human immunodeficiency virus 1, amino acid sequence, article, blood sampling, carboxy terminal sequence, female, gene amplification, gene sequence, human, Human immunodeficiency virus 1, Human immunodeficiency virus infected patient, male, nonhuman, nucleotide sequence, phylogeny, priority journal, promoter region, reverse transcription polymerase chain reaction, sequence analysis, South Africa, virus gene, virus strain, classification, cluster analysis, DNA sequence, genetics, genotype, Human immunodeficiency virus infection, isolation and purification, missense mutation, molecular genetics, protein motif, sequence homology, virology, Amino Acid Motifs, Cluster Analysis, Conserved Sequence, Genotype, HIV Infections, HIV-1, Humans, Molecular Sequence Data, Mutation, Missense, Phylogeny, Sequence Analysis, DNA, Sequence Homology, Amino Acid, South Africa, vpr Gene Products, Human Immunodeficiency Virus
Citation
AIDS Research and Human Retroviruses
25
3
25
3