Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization

Smith A.S.G. ; Rawlings D.E. (1998)

Article

The pasABC genes of the proteic plasmid addiction system of broad-host- range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100- fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-β-D-thiogalactopyranoside (IPTG)- regulated tac promoter, they were unable to stabilize a heterologous test plasmid.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/12202
This item appears in the following collections: