A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 represser gene
Date
1996
Authors
Lambrechts M.G.
Sollitti P.
Murmur J.
Pretorius I.S.
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Abstract
Transcription of the three unlinked, homologous STA1-3 glucoamylase-encoding genes, involved in starch degradation by Saccharomyces cerevisiae, was previously shown to be down-regulated by the presence of STA10, acting via three upstream repression sequence regions that were identified in the STA2 promoter. Here we report the cloning and characterization of a putative transcriptional activator gene, MSS10 (multicopy suppressor of STA10), which, when present in multiple copies, overcomes STA10 repression. Deletion of MSS10, located on chromosome XV, resulted in media-specific extinction of glucoamylase synthesis. The nucleotide sequence of MSS10 is identical to three other genes from S. cerevisiae identified as: FUP1, a gene that enhances iron-limited growth; PHD2, a gene identified for its ability to induce pseudohyphal growth in diploid cells grown on nitrogen-limited media; and MSN1, a gene encoding a transcriptional activator involved in invertase regulation.
Description
Keywords
fungal protein, glucan 1,4 alpha glucosidase, starch, transcription factor, fungal DNA, article, carbohydrate metabolism, controlled study, enzyme synthesis, fungal genetics, gene deletion, gene number, molecular cloning, nonhuman, nucleotide sequence, priority journal, promoter region, repressor gene, saccharomyces cerevisiae, sequence homology, suppressor gene, transcription regulation, biosynthesis, diploidy, enzymology, fungal gene, gene expression, genetics, heterozygote, metabolism, multigene family, regulator gene, restriction mapping, Saccharomyces cerevisiae, transactivation, Cloning, Molecular, Diploidy, DNA, Fungal, Gene Expression, Genes, Fungal, Genes, Regulator, Genes, Suppressor, Glucan 1,4-alpha-Glucosidase, Heterozygote, Multigene Family, Restriction Mapping, Saccharomyces cerevisiae, Starch, Trans-Activation (Genetics)
Citation
Current Genetics
29
6
29
6