Inhibition of transcription by adriamycin is a consequence of the loss of negative superhelicity in DNA mediated by topoisomerase II
Date
1990
Authors
Tarr M.
Van Helden P.D.
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Abstract
Adriamycin is commonly used as a chemotherapeutic agent and is known to intercalate into the major groove of DNA and inhibit DNA and RNA synthesis. Results presented in this communication suggest that adriamycin affects topoisomerase cleavage of DNA. The resultant change in negative superhelicity (decrease) is responsible for the decrease in transcription. This process is not dependent on the continued presence of adriamycin. The reaction between topoisomerases, DNA and adriamycin is dose-dependent. The results help to explain the relatively enhanced cytotoxicity of this drug to tumor cells.
Description
Keywords
dna topoisomerase, doxorubicin, article, cytotoxicity, dna supercoiling, dna transcription, priority journal, Animal, DNA Topoisomerases, Type I, DNA Topoisomerases, Type II, DNA, Superhelical, Doxorubicin, Escherichia coli, In Vitro, Nucleic Acid Conformation, Oocytes, Plasmids, Templates, Genetic, Transcription, Genetic, Xenopus laevis
Citation
Molecular and Cellular Biochemistry
93
2
93
2