Towards an optimum spore immobilisation strategy using Phanerochaete chrysosporium, reverse filtration and ultrafiltration membranes
Date
2004
Authors
Govender S.
Jacobs E.P.
Leukes W.D.
Odhav B.
Pillay V.L.
Journal Title
Journal ISSN
Volume Title
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Abstract
An externally unskinned polysulfone ultrafiltration capillary membrane was used as an immobilisation matrix for the white rot basidiomycete Phanerochaete chrysosporium ME446. In this study, different membrane bioreactor configurations, scanning electron microscopy, atomic force microscopy and enzyme assays were used to characterise the spatial distribution of spores after immobilisation by reverse filtration and document the subsequent growth phases. A single capillary membrane bioreactor was also used to develop and evaluate a spore immobilisation technique by modifying conventional bioreactor configurations for reverse filtration onto the external surface of the membrane. Results indicate that spore immobilisation was dependent on a differential pressure across the tube wall and the fluid flow pattern in the tube, thus influencing the spatial distribution of spores along the surface and within the microvoids in the membrane wall. The resultant radial and lateral biofilm development using this reverse filtration approach promoted uniform biomass development, which favoured manganese peroxidase production. © 2004 Elsevier B.V. All rights reserved.
Description
Keywords
Atomic force microscopy, Biofilms, Biomass, Bioreactors, Filtration, Matrix algebra, Membranes, Optimization, Scanning electron microscopy, Ultrafiltration, Immobilisation matrix, Reverse filtration, Spore immobilisation strategy, Polysulfones, manganese peroxidase, mechanical property, membrane, polysulfone, ultrafiltration, article, atomic force microscopy, biofilm, biomass, bioreactor, capillary wall, enzyme assay, enzyme synthesis, fluid flow, fungus growth, fungus spore, immobilized cell, membrane filter, nonhuman, Phanerochaete, pressure, priority journal, scanning electron microscopy, tube, ultrafiltration
Citation
Journal of Membrane Science
238
02-Jan
238
02-Jan