Recovery of trypsin inhibitor and soy milk protein concentration by dynamic filtration
Date
2006
Authors
Akoum O.
Richfield D.
Jaffrin M.Y.
Ding L.H.
Swart P.
Journal Title
Journal ISSN
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Abstract
This paper investigates the feasibility of producing a soy milk fraction enriched in trypsin inhibitor (STI) which could serve as a feed material for chromatographic purification of STI. This STI is also a soy antinutritional factor which is valuable for its medical and scientific applications. We have used a shear-enhanced filtration system with a disk rotating at high speed near the membrane. Stabilized permeate fluxes at 2500 rpm, with a disk equipped with vanes and a transmembrane pressure (TMP) of 67 kPa were 92 L h-1 m-2 with a 50 kDa MWCO PES membrane against 60 L h-1 m-2 with a 300 kDa one, under the same conditions. STI rejection by the 50 kDa membrane was 98.6%. With a smooth disk at a TMP of 107 kPa, fluxes fell to 30 L h-1 m-2 for both membranes, and rejection remained close to 98% for the 50 kDa membrane. During concentration tests, the permeate flux obeyed the logarithmic decay with concentration factor (CF), with a theoretical maximum CF of 4.85. © 2006 Elsevier B.V. All rights reserved.
Description
Keywords
Chromatography, Concentration (process), Enzyme inhibition, Enzymes, Mechanical permeability, Proteins, Purification, Rotating disks, Ultrafiltration, Concentration factor (CF), Dynamic ultrafiltration, Soy milk, Trypsin inhibitor, Permselective membranes, milk protein, trypsin inhibitor, Chromatography, Concentration (process), Enzyme inhibition, Enzymes, Mechanical permeability, Permselective membranes, Proteins, Purification, Rotating disks, Ultrafiltration, article, chromatography, concentration (parameters), experimentation, feasibility study, filtration, heart protection, mathematical analysis, molecular weight, priority journal, purification, soybean milk, theory, velocity
Citation
Journal of Membrane Science
279
02-Jan
279
02-Jan