ITEM VIEW

Enterocin 012, a bacteriocin produced by Enterococcus gallinarum isolated from the intestinal tract of ostrich

dc.contributor.authorJennes W.
dc.contributor.authorDicks L.M.T.
dc.contributor.authorVerwoerd D.J.
dc.date.accessioned2011-05-15T15:59:25Z
dc.date.available2011-05-15T15:59:25Z
dc.date.issued2000
dc.identifier.citationJournal of Applied Microbiology
dc.identifier.citation88
dc.identifier.citation2
dc.identifier.issn13645072
dc.identifier.other10.1046/j.1365-2672.2000.00979.x
dc.identifier.urihttp://hdl.handle.net/10019.1/11168
dc.description.abstractEnterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against Ent. faecalis, Lactobacillus acidophilus, Lact. sake, Listeria innocua, Propionibacterium acidipropionici, Propionibacterium sp., Clostridium perfringens, Pseudomonas aeruginosa and Salmonella typhimurium. One of the four pathogenic strains of Escherichia coli isolated from the intestinal tract of ostrich was inhibited by enterocin 012. No antimicrobial activity was recorded against Bacillus cereus, Cl. sporogenes, Cl. tyrobutyricum, Leuconostoc cremoris, Pediococcus pentosaceus, Staphylococcus carnosus and Streptococcus thermophilus. Enterocin 012 was resistant to treatment with lysozyme, catalase, lipase and papain, but sensitive to Proteinase K, α-chymotrypsin, trypsin and pepsin. Treatment of enterocin 012 with gastric juice from the duodenum resulted in a 50% loss of antibacterial activity. Half of the activity was lost when incubated at 80°C for 30 min, or when kept overnight at a pH of 1.0-5.0 and pH 11.0 and 12.0, respectively. Enterocin 012 production started in mid- logarithmic growth and reached a maximum of 800 AU ml-1, but increased further to 1600 AU ml-1 in the stationary growth phase. The peptide is approximately 3.4 kDa in size, as determined after partial purification with Amberlite XAD-1180 and ammonium sulphate precipitation, followed by tricinesodium dodecyl sulphate-polyacrylamide gel electrophoresis. The mechanism of antimicrobial activity against Lact. sake LMG 13558 is bactericidal and caused cell lysis of active growing cells.
dc.subjectbacteriocin
dc.subjectchymotrypsin A
dc.subjectpepsin A
dc.subjectproteinase K
dc.subjecttrypsin
dc.subjectantibacterial activity
dc.subjectarticle
dc.subjectbacterial growth
dc.subjectbactericidal activity
dc.subjectbacterium isolation
dc.subjectcytolysis
dc.subjectEnterococcus gallinarum
dc.subjectintestine
dc.subjectnonhuman
dc.subjectostrich
dc.subjectAnimals
dc.subjectBacteriocins
dc.subjectEnterococcus
dc.subjectGram-Positive Bacteria
dc.subjectIntestines
dc.subjectMicrobial Sensitivity Tests
dc.subjectStruthioniformes
dc.subjectBacillus cereus
dc.subjectBacteria (microorganisms)
dc.subjectClostridium perfringens
dc.subjectClostridium sporogenes
dc.subjectClostridium tyrobutyricum
dc.subjectEnterococcus faecalis
dc.subjectEnterococcus gallinarum
dc.subjectEscherichia coli
dc.subjectLactobacillus acidophilus
dc.subjectLactobacillus sakei
dc.subjectLeuconostoc
dc.subjectLeuconostoc mesenteroides subsp. cremoris
dc.subjectListeria innocua
dc.subjectPediococcus pentosaceus
dc.subjectPosibacteria
dc.subjectPropionibacterium
dc.subjectPropionibacterium acidipropionici
dc.subjectPseudomonas
dc.subjectPseudomonas aeruginosa
dc.subjectSalmonella typhimurium
dc.subjectStaphylococcus carnosus
dc.subjectStreptococcus thermophilus
dc.subjectStruthio camelus
dc.subjectTyphimurium
dc.titleEnterocin 012, a bacteriocin produced by Enterococcus gallinarum isolated from the intestinal tract of ostrich
dc.typeArticle
dc.description.versionArticle


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

ITEM VIEW