Adhesion of Lactobacillus plantarum 423 and Lactobacillus salivarius 241 to the intestinal tract of piglets, as recorded with fluorescent in situ hybridization (FISH), and production of plantaricin 423 by cells colonized to the ileum
Date
2006
Authors
Mare L.
Wolfaardt G.M.
Dicks L.M.T.
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Abstract
Aims: To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model. Methods and Results: Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combination, were administered to 1-, 14- and 28-day-old (postweaned) piglets. According to results obtained by fluorescent in situ hybridization (FISH), Lact. plantarum 423 adhered strongly to the ileum and posterior colon and Lact. salivarius 241 to the duodenum in preweaned piglets. High numbers of strain 241 were recorded in the duodenum and posterior colon of postweaned piglets, whereas strain 423 remained localized to the ileum. Lowering in Enterococcus faecalis cell numbers were recorded when preweaned piglets were challenged with strain 241. Plantaricin 423 was produced for 96 h in the ileum section of a gastro-intestinal model. Conclusions: Lactobacillus plantarum 423 and Lact. salivarius 241 adhere to different sections of the intestinal tract, depending on the piglet's age. Ent. faecalis were inhibited in vivo, probably by plantaricin 423. Significance and Impact of the Study: Fluorescent in situ hybridization proved valuable in the detection of probiotic bacteria adhered to the intestine. This is the first report of bacteriocin production in a model simulating the porcine gastro-intestinal tract. © 2006 The Society for Applied Microbiology.
Description
Keywords
bacteriocin, probiotic agent, bacterial disease, colonization, digestive system, pig, article, bacterial strain, cell count, colon, duodenum, Enterococcus faecalis, fluorescence in situ hybridization, ileum, intestine, Lactobacillus plantarum, Lactobacillus salivarius, nonhuman, swine, Animals, Anti-Bacterial Agents, Bacterial Adhesion, Bacteriocins, Colon, Colony Count, Microbial, Duodenum, Enterococcus faecalis, Gastrointestinal Tract, Ileum, In Situ Hybridization, Fluorescence, Jejunum, Lactobacillus, Lactobacillus plantarum, Probiotics, Species Specificity, Swine, Bacteria (microorganisms), Enterococcus faecalis, Lactobacillus plantarum, Lactobacillus salivarius, Suidae, Sus scrofa
Citation
Journal of Applied Microbiology
100
4
100
4