The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat

Storbeck, Karl-Heinz (Stellenbosch : Stellenbosch University, 2008-12)

Thesis (PhD (Biochemistry))--Stellenbosch University, 2008.

Thesis

This study describes: • the cloning and sequencing of cytochrome P450 17 -hydroxylase/17,20 lyase (CYP17), 3 -hydroxysteroid dehydrogenase (3 HSD) and cytochrome b5 from the South African Angora goat; • the identification of two CYP17 genes encoding two unique CYP17 isoforms in the Angora goat; • the development of a UPLC-APCI-LC method for the separation and quantification of seven adrenal steroids; • the characterisation of the enzymatic activity of the two Angora CYP17 isoforms expressed in non-steroidogenic COS-1 cells. The Km and Vvalues for the metabolism of pregnenolone and progesterone were determined; • the development of a rapid and accurate real-time PCR genotyping test for CYP17 in Angora goats. Three unique genotypes were identified; • the determination of blood cortisol levels upon the stimulation of the HPAaxis by intravenous insulin injection in the three Angora goat genotypes.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/1101
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