Confirmation of the effectiveness and genetic positions of disease resistance loci in ‘Kishmish Vatkana’ (Ren1) and ‘Villard Blanc’ (Ren3 and Rpv3)
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
South African Society for Enology and Viticulture
Abstract
This study aimed to validate the effectiveness and to genetically characterise the fungal disease resistance genes of ‘Kishmish Vatkana’ and ‘Villard Blanc’ in South Africa using microsatellite (SSR) markers and
a Quantitative Trait Loci (QTL) approach. An F1 ‘Sunred Seedless’ × ‘Kishmish Vatkana’ cross was used to generate a partial linkage map for chromosome 13 known to harbour the Ren1 powdery mildew locus
of ‘Kishmish Vatkana’. The effectiveness of this locus was validated, explaining between 44.8% and 57.7% of the observed phenotypic variance. An F1 ‘Villard Blanc’ × ‘G1-6604’ cross was used to generate partial linkage maps for chromosomes 15 and 18, reported to harbour fungal resistance genes of ‘Villard Blanc’. The powdery mildew QTL (Ren3) was validated on chromosome 15 of ‘Villard Blanc’, which explained
between 18.9% and 23.9% of the phenotypic variance observed. The downy mildew resistance QTL on chromosome 18 (Rpv3) of ‘Villard Blanc’ was also confirmed, and it explained between 19.1% and 21.2%
of the phenotypic variance observed. This molecular information and individual sources of resistance have already been implemented in the marker-assisted selection (MAS) and gene pyramiding efforts of the table
grape breeding program of the Agricultural Research Council (ARC) Infruitec-Nietvoorbij.
Description
CITATION: Veikondis, R. et al. 2018. Confirmation of the effectiveness and genetic positions of disease resistance loci in ‘Kishmish Vatkana’ (Ren1) and ‘Villard Blanc’ (Ren3 and Rpv3). South African Journal of Enology and Viticulture, 39(2):185-195, doi:10.21548/39-2-2685.
The original publication is available at https://www.journals.ac.za/index.php/sajev
The original publication is available at https://www.journals.ac.za/index.php/sajev
Keywords
Powdery mildew diseases -- South Africa, Fungal diseases of plants -- Genetics, Gene mapping, Genetics -- Statistical methods, Genotype-environment interaction -- Analysis, Grapes -- Breeding, Kishmish Vatkana -- Genome mapping, Villard Blanc -- Genome mapping
Citation
Veikondis, R. et al. 2018. Confirmation of the effectiveness and genetic positions of disease resistance loci in ‘Kishmish Vatkana’ (Ren1) and ‘Villard Blanc’ (Ren3 and Rpv3). South African Journal of Enology and Viticulture, 39(2):185-195, doi:10.21548/39-2-2685.