Investigation of thiol levels in young commercial South African Sauvignon Blanc and Chenin Blanc wines using propiolate derivatization and GC-MS/MS
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
South African Society for Enology and Viticulture
Abstract
In this work, the ethyl propiolate method for analysing thiols in white wine by GC-MS, originally proposed by Herbst-Johnstone et al.) (2013), has been adapted to GC-MS/MS and has been validated. The method performance has shown improvement in terms of sensitivity (limit of detection, LOD) and of the number of compounds measured. In addition to 3-mercaptohexanol (3MH), 3-mercaptohexyl acetate (3MHA), and 4-mercapto-4-methylpentan-2-one (4MMP), the adapted method can also measure 2-furanmethanethiol (FMT) and makes use of a commercially-available internal standard (IS), 4-methoxy-2-methyl-2-butanethiol (4M2M2B, IS). The proposed method was applied to determine thiol levels in young commercial South African Sauvignon and Chenin Blanc wines. The samples (n=20 for each cultivar) were chosen according to a high frequency of the typical descriptors associated with this class of impact compounds. 3MH was found at 178-904 ng/L and 99-1124 ng/L, and 3MHA at 23-151 ng/L and 5-253 ng/L in Sauvignon and Chenin Blanc respectively. 4MMP was present in Sauvignon Blanc in concentrations up to 21.9 ng/L, but in none of the Chenin Blanc samples.
Description
CITATION: Coetzee, C. et al. 2018. Investigation of thiol levels in young commercial South African Sauvignon Blanc and Chenin Blanc wines using propiolate derivatization and GC-MS/MS. South African Journal of Enology and Viticulture, 39(2):180-184, doi:10.21548/39-2-2683.
The original publication is available at https://www.journals.ac.za/index.php/sajev
The original publication is available at https://www.journals.ac.za/index.php/sajev
Keywords
Thiols -- Analysis, Sauvignon blanc (Wine) -- South Africa, Chenin Blanc wines -- South Africa, White wines, Wine and wine making -- Chemistry, Wine -- Flavor and odor
Citation
Coetzee, C. et al. 2018. Investigation of thiol levels in young commercial South African Sauvignon Blanc and Chenin Blanc wines using propiolate derivatization and GC-MS/MS. South African Journal of Enology and Viticulture, 39(2):180-184, doi:10.21548/39-2-2683.