The development of a medical biomarker test to aid in rapid screening for the presence of infectious diseases

Bradford, Dean John (2019-04)

Thesis (MEng)--Stellenbosch University, 2019.

Thesis

ENGLISH ABSTRACT: The widespread threat of antimicrobial resistance poses a major problem worldwide. A low-cost rapid biomarker test to aid in the screening of infectious diseases could potentially play a major role in promoting antimicrobial stewardship, thus combating antimicrobial resistance. Here, the prototype development of such a test is reported. C-reactive protein (CRP) and procalcitonin (PCT) were the chosen biomarkers for the test, based on recommendations from literature. Organic electrochemical transistors (OECTs) were chosen for the sensing platform, and were subsequently designed and fabricated using a desktop inkjet printer. The OECT was made sensitive to the target biomarkers by immobilising antibodies, as biorecognition elements, onto the sensor surface. The sensor was then characterised and tested using a National Instruments data acquisition device (DAQ) controlled by a series of LabVIEW programs. The CRP sensors displayed sensitivity to CRP concentrations between 0.3125 ug/mL and 10 ug /mL, whilst the PCT sensors showed no discernible response to the PCT protein. Further work is recommended to enable exact quantification of CRP concentrations, as well as enable the correct detection of PCT proteins.

AFRIKAANSE OPSOMMING: Wydverspreide antimikrobiese weerstand is 'n wêreldwye probleem. 'n Goedkoop, vinnige biomerker toets, om te help met die sifting van aansteeklike siektes, kan dus 'n groot rol in die bevordering van antimikrobiese weerstand speel. In hierdie studie word die ontwikkeling van so 'n prototiepe aangemeld. C-reactive protein (CRP) en procalcitonin (PCT) is gekies as biomerkers na aanleiding van aanbevelings vanuit die literatuur studie. Organiese elekrochemiese transistors (OECTs) is gekies vir die sensor platform, en is ontwerp om deur 'n goedkoop huishoudelike drukker vervaardig te word. Die OECT is sensitief tot die teiken biomerkers by ge- ïmmobiliseerde teenligaampies as bio-erkenning elemente, op die sensor oppervlak te gebruik. Die sensor is gekarakteriseer en getoets met 'n National Instruments (NI) data verkrygings toestel (DAQ) en is gekontroleer deur LabVIEW sagteware. Die CRP sensors het sensitiwiteit vir CRP konsentrasies tussen 0.3125 ug/mL en 10 ug/mL gewys, terwyl die PCT sensors geen waarneembare reaksie tot PCT protein gewys het nie. Verdere navorsing om die presiese kwantifisering van CRP konsentrasies aan te dui en die effektiewe opsporing van PCT proteïne vergemaklik word aanbeveel.

Please refer to this item in SUNScholar by using the following persistent URL: http://hdl.handle.net/10019.1/106126
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