Genotyping of Mycobacterium tuberculosis with additional markers enhances accuracy in epidemiological studies
Date
1996
Authors
Warren R.
Richardson M.
Sampson S.
Hauman J.H.
Beyers N.
Donald P.R.
Van Helden P.D.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Two highly polymorphic Mycobacterium tuberculosis genomic domains, characterized by hybridization to the oligonucleotide (GTG)5, were identified as potential DNA fingerprinting probes. These domains were cloned [pMTB484(1) and pMTB484(2K4), respectively] and shown to be useful for genotype analysis by Southern blotting. These probes were used to genotype geographically linked strains of M. tuberculosis previously shown to have identical IS6110 fingerprints. Subsequent DNA fingerprints generated with MTB484(1) and MTB484(2K4) showed a high degree of polymorphism, allowing subclassification of IS6110-defined clusters into composites of smaller clusters and unique strains. Correlation of the molecular data with patient interviews and clinical records confirmed the sensitivity of these probes, as contacts were established only within subclusters. These findings demonstrate the requirement for multiple probes to accurately classify M. tuberculosis strains, even those with high copy numbers of IS6110. The enhanced accuracy of strain typing should, in turn, further our understanding of the epidemiology of tuberculosis.
Description
Keywords
antibody specificity, article, diagnostic accuracy, dna fingerprinting, gene cluster, genetic recombination, genotype, human, human cell, mycobacterium tuberculosis, priority journal, protein domain, restriction fragment length polymorphism, tandem repeat, tuberculosis, Bacterial Typing Techniques, Biological Markers, DNA Fingerprinting, DNA, Bacterial, Genome, Bacterial, Molecular Probe Techniques, Mycobacterium tuberculosis, Mycobacterium, Mycobacterium tuberculosis
Citation
Journal of Clinical Microbiology
34
9
34
9