Analysis for a limited number of gene codons can predict drug resistance of Mycobacterium tuberculosis in a high-incidence community
Date
2001
Authors
Van Rie A.
Warren R.
Mshanga I.
Jordaan A.M.
Van Der Spuy G.D.
Richardson M.
Simpson J.
Gie R.P.
Enarson D.A.
Beyers N.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Correct and rapid diagnosis is essential in the management of multidrug-resistant tuberculosis (MDR-TB). In this population-based study of 61 patients with drug-resistant tuberculosis, we evaluated the frequency of mutations and compared the performance of genotypic (mutation analysis by dot blot hybridization) and phenotypic (indirect proportion method) drug resistance tests. Three selected codons (rpoB531, rpoB526, and katG315) allowed identification of 90% of MDR-TB cases. Ninety percent of rifampin, streptomycin, and ethambutol resistance and 75% of isoniazid resistance were detected by screening for six codons: rpoB531, rpoB526, rrs-513, rpsL43, embB306, and katG315. The performance (reproducibility, sensitivity, and specificity) of the genotypic method was superior to that of the routine phenotypic method, with the exception of sensitivity for isoniazid resistance. A commercialized molecular genetic test for a limited number of target loci might be a good alternative for a drug resistance screening test in the context of an MDR "DOTS-plus" strategy.
Description
Keywords
ethambutol, isoniazid, rifampicin, streptomycin, adolescent, adult, article, codon, dot hybridization, female, genotype, human, major clinical study, male, multidrug resistance, mutation rate, Mycobacterium tuberculosis, phenotype, priority journal, school child, tuberculosis, Bacterial Proteins, Cluster Analysis, Codon, DNA-Directed RNA Polymerases, Drug Resistance, Multiple, Genotype, Humans, Incidence, Microbial Sensitivity Tests, Mutation, Mycobacterium tuberculosis, Peroxidases, Phenotype, Polymorphism, Restriction Fragment Length, South Africa, Sputum, Tuberculosis, Actinobacteria (class), Mycobacterium, Mycobacterium tuberculosis, uncultured actinomycete
Citation
Journal of Clinical Microbiology
39
2
39
2